Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy.
Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy.
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DOI：
10.1038/s41598-017-00995-y
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发表时间：
2017-04-12
影响因子：
4.6
通讯作者：
Takamatsu T
中科院分区：
文献类型：
--
作者：
Kumamoto Y;Harada Y;Tanaka H;Takamatsu T
拉曼光谱可以无标记、微创和准确地检测周围神经。然而，传统的拉曼成像技术在测量大面积样品时非常耗时。建立一种快速获取周围神经纤维束的空间分布的方法是拉曼光谱走向临床外科应用的关键一步。在这里，我们提出了一种用于快速周围神经成像的多点拉曼光谱技术。仅在5 秒内，就在大鼠周围神经束和毗邻结缔组织上获得了32个点的光谱。主成分回归和频谱判别分析表明，神经检测的灵敏度为85.8%，特异度为96.0%，准确度为90.8%。在158条周围神经中，有152条(96.2%)神经阳性预测点与总测量点之比在0.4以上，而在120条结缔组织中，有119条(99.2%)神经阳性预测点与总测量点之比小于0.4。基于该比值和样本的明视场图像，实现了周围神经的精确可视化。结果表明，多点拉曼光谱技术能够快速、准确地对周围神经进行成像。
Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired. Principal component regression and discriminant analysis of spectra revealed that the sensitivity, specificity and accuracy for nerve detection were 85.8%, 96.0%, and 90.8%, respectively. Of 158 peripheral nerves, 152 (96.2%) showed ratio of the number of nerve-positive prediction points to the total measurement points being 0.4 or larger, whereas 119 (99.2%) connective tissues among 120 showed ratio smaller than 0.4. Based on the ratio and a bright-field image of the sample, accurate visualization of peripheral nerves was implemented. The results indicated that the multipoint Raman spectroscopic technique is capable of rapid and accurate peripheral nerve imaging.