Legionella pneumophila contains a type II general secretion pathway required for growth in amoebae as well as for secretion of the Msp protease
Legionella pneumophila contains a type II general secretion pathway required for growth in amoebae as well as for secretion of the Msp protease
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DOI：
10.1128/iai.67.7.3662-3666.1999
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发表时间：
1999-07-01
影响因子：
3.1
通讯作者：
Shuman, HA
中科院分区：
文献类型：
--
作者：
Hales, LM;Shuman, HA
我们报告了一组嗜肺军团菌基因的鉴定，这些基因编码的产物与革兰氏阴性细菌的II型一般分泌途径的蛋白同源。含有其中两个基因的缺失替代突变的菌株不能分泌MSP蛋白酶。该菌株不能在自由生活的卡氏阿米巴内繁殖，但能够杀死HL-60来源的巨噬细胞。因为MSP不是阿米巴生长所必需的，所以对阿米巴生长至关重要的其他蛋白质很可能是通过这一途径分泌的。
We report the identification of a set of Legionella pneumophila genes that encode products with homology to proteins of the type II general secretion pathway of gram-negative bacteria. A strain containing a deletion-substitution mutation of two of these genes was unable to secrete the Msp protease. This strain was unable to multiply within the free-living amoeba Acanthamoeba castellanii yet was able to kill HL-60-derived macrophages. Because Msp is not required for growth in amoebae, other proteins which are important for growth in amoebae are likely secreted by this pathway.