A Tick-borne Encephalitis Virus Vaccine Based on the European Prototype Strain Induces Broadly Reactive Cross-neutralizing Antibodies in Humans
A Tick-borne Encephalitis Virus Vaccine Based on the European Prototype Strain Induces Broadly Reactive Cross-neutralizing Antibodies in Humans
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DOI：
10.1093/infdis/jir122
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发表时间：
2011-06-01
影响因子：
6.4
通讯作者：
Kreil, Thomas R.
中科院分区：
文献类型：
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作者：
Orlinger, Klaus K.;Hofmeister, Yvonne;Kreil, Thomas R.
在人类接种蜱传脑炎病毒（TBEV）疫苗后，分析了欧洲、远东和西伯利亚TBEV亚型病毒与鄂木斯克出血热病毒（OHFV）之间的交叉中和程度。使用西尼罗河病毒（WNV）骨架作为载体构建编码所有亚型内的代表性病毒的TBEV表面蛋白和OHFV的杂合病毒。这些病毒允许在中和试验中进行无偏倚的头对头比较，因为它们表现出表面蛋白来源的TBEV毒株的抗原特征，并在细胞培养中显示出等同的生物学特性。对来自TBEV疫苗试验的人血清样品进行了分析，发现对欧洲、远东和西伯利亚亚型病毒的中和抗体滴度相当，表明对这些TBEV毒株的交叉保护作用同样有效，对更远的相关病毒（如OHFV）的中和能力有所降低，但仍具有保护性。
After vaccination of humans with tick-borne encephalitis virus (TBEV) vaccine, the extent of cross-neutralization between viruses of the European, Far Eastern, and Siberian subtypes of TBEV and Omsk hemorrhagic fever virus (OHFV) was analyzed. Hybrid viruses that encode the TBEV surface proteins for representative viruses within all subtypes, and OHFV, were constructed using the West Nile virus (WNV) backbone as vector. These viruses allow for unbiased head-to-head comparison in neutralization assays because they exhibit the antigenic characteristics of the TBEV strains from which the surface proteins were derived and showed equivalent biologic properties in cell culture. Human serum samples derived from a TBEV vaccine trial were analyzed and revealed comparable neutralizing antibody titers against European, Far Eastern, and Siberian subtype viruses, indicating equally potent cross-protection against these TBEV strains and a somewhat reduced but still protective neutralization capacity against more distantly related viruses, such as OHFV.