Fractionation of dextrin by gradient polyethylene glycol precipitation
Fractionation of dextrin by gradient polyethylene glycol precipitation
复制标题
通过梯度聚乙二醇沉淀分离糊精
DOI：
10.1016/j.chroma.2016.01.021
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发表时间：
2016
影响因子：
4.1
通讯作者：
Yaoqi Tian
中科院分区：
文献类型：
--
作者：
Xiuting Hu;Chengmei Liu;Zhengyu Jin;Yaoqi Tian
本工作旨在开发一种新的方法，称为梯度聚乙二醇（PEG）沉淀，分级糊精具有较窄的分子量分布的馏分。这种方法是基于PEG和糊精在水溶液中的不相容性;这种不相容性与糊精的分子量呈正相关。从理论上讲，糊精可以通过逐渐向糊精溶液中加入PEG以分子量降序沉淀。以分子量分散度（DM）为指标，考察了PEG分子量及其分布对糊精分级的影响。利用PEG对糊精进行分级，可以得到分子量不同的低DM级分。PEG 2000、PEG 4000、PEG 6000、PEG 10000和PEG 20000得到的组分的平均DM分别为1.471、1.352、1.286、1.453和2.028，而母体糊精的DM为2.052。结果表明，PEG 6000是最佳的沉淀剂，而PEG 20000不适合于糊精的分级。此外，窄分布的PEG导致最佳分级结果。因此，PEG梯度沉淀法是一种分离糊精的有效方法。此外，应选择合适分子量的窄分布PEG以获得上级分级结果。
This work aimed at developing a novel approach, named gradient polyethylene glycol (PEG) precipitation, to fractionate dextrin into fractions with narrower molecular weight distribution. This approach was based on the incompatibility between PEG and dextrin in aqueous solution; this incompatibility is positively correlated with the molecular weight of dextrin. Theoretically, dextrin can be precipitated in descending order of molecular weight by the gradual addition of PEG into the dextrin solution. Specifically, this study investigated the effects of molecular weight and its distribution of PEG on dextrin fractionation with the molecular-weight dispersity (DM) as index. The parent dextrin could be fractionated by PEG into several fractions with different molecular weights and lowerDM. The averageDMof fractions obtained by PEG2000, PEG4000, PEG6000, PEG10000, and PEG20000 was 1.471, 1.352, 1.286, 1.453, and 2.028, respectively, while theDMof the parent dextrin was 2.052. These data suggest that PEG6000 was the optimal precipitant, while PEG20000 was unsuitable for fractionating dextrin. Furthermore, narrowly-distributed PEG resulted in optimum fractionation results. Therefore, gradient PEG precipitation is an efficient method for fractionating dextrin. Additionally, narrowly-distributed PEG of suitable molecular weight should be selected to obtain superior fractionation results.