Genetic diversity analysis in Vicia species using retrotransposon-based SSAP markers
Genetic diversity analysis in Vicia species using retrotransposon-based SSAP markers
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DOI：
10.1007/s00438-007-0261-x
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发表时间：
2007-10-01
影响因子：
3.1
通讯作者：
Flavell, Andrew J.
中科院分区：
文献类型：
--
作者：
Martin Sanz, Alberto;Gilsanz Gonzalez, Susana;Flavell, Andrew J.
比较了 12 种不同的 Ty1-copia 和 Ty3-gypsy 组 LTR 反转录转座子在两种农业上重要的蚕豆物种的 SSAP 标记开发中的有用性。三种反转录转座子 PDR1、Tps19 和 Tvf4 在蚕豆和纳博山蚕中产生了有用的 SSAP 标记系统。另外，Tvf1 是 V. narbonensis 中 SSAP 标记的良好来源。将优化的 SSAP 标记系统应用于两种不同的蚕豆种质资源集的分析。在 56 个纳博蚕样品中对 202 个多态性 Tvf1 SSAP 标记进行了评分，在 20 个蚕豆样品中对源自其他三种最有用的反转录转座子的 196 个多态标记进行了评分。然后使用标记数据构建系统发育树。这两个物种的树木往往都表现出长枝长度，而精细结构相当少。一些 V. narbonensis 种质按地理起源聚集，但许多则不然，并且一个特定的地理区域通常由树中的多个不同群体代表，这表明 V. narbonensis 的多样性具有跨越其当前地理范围的深刻而古老的结构。蚕豆种质的树也显示出与地理起源非常有限的聚类，并且该物种的多样性和基于形态的分类分组之间没有明显的相关性。
Twelve different Ty1-copia and Ty3-gypsy group LTR retrotransposons were compared for their usefulness in SSAP marker development in two agriculturally important Vicia species. Three of the retrotransposons, PDR1, Tps19 and Tvf4, yielded useful SSAP marker systems in V. faba, and V. narbonensis. Another, Tvf1 was a good source of SSAP markers in V. narbonensis alone. The optimized SSAP marker systems were applied to the analysis of two diverse Vicia germplasm sets. Two hundred and two polymorphic Tvf1 SSAP markers were scored in 56 V. narbonensis samples and 196 polymorphic markers derived from the other three most useful retrotransposons were scored in a collection of 20 V. faba samples. The marker data were then used to construct phylogenetic trees. The trees for both species tend to show long-branch lengths, with rather little fine structure. Some V. narbonensis accessions cluster by geographical origin but many do not and a given geographical region is often represented by multiple diverse groups in the tree, suggesting a deep and ancient structure for the diversity of V. narbonensis that spans its current geographic range. The tree for the V. faba accessions also shows very limited clustering with geographical origin and no obvious correlation between diversity and morphology-based taxonomic groupings for the species.