Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.)
Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.)
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开发新的基于 PCR 的黑麦染色体臂特异性标记（Secale Greeke L.）
DOI：
10.1139/gen-2015-0154
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发表时间：
2016-03-01
期刊：
影响因子：
3.1
通讯作者：
Fu, Shu-lan
中科院分区：
文献类型：
--
作者：
Qiu, Ling;Tang, Zong-xiang;Fu, Shu-lan
基于pcr的黑麦（Secale cereale L.）染色体特异性标记，有助于在小麦（Triticum aestivum L.）育种中有效利用黑麦的优良基因。利用特异性长度扩增片段测序（SLAF-seq）技术，构建了578个基于pcr的黑麦特异性标记，其中76个标记在Kustro、Imperial和King II黑麦中表现出不同的多态性。利用一组小麦-黑麦单端体附加系和13个来自T. aestivum L的单端体附加系，分别在Kustro的单个染色体和染色体臂上定位了427个和387个标记。‘绵阳11号’× S. cereale L。“Kustro”。此外，还获得了两组小麦-黑麦二体附加系，这两组附加系分别来自中国春季小麦和帝王小麦。采用‘Holdfast’ x S. cereale L. var. King II，检测427个标记的染色体特异性。281个标记的染色体位置在3组小麦-黑麦附加系中是一致的。本研究开发的标记可以在小麦背景下识别黑麦染色体的特定片段，加快黑麦染色体上的优良基因在小麦育种中的利用。
PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat-rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. 'Mianyang11' x S. cereale L. 'Kustro'. In addition, two sets of wheat-rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring x S. cereale L. var. Imperial and T. aestivum L. 'Holdfast' x S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat-rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.