Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes
Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes
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DOI：
10.1128/jb.184.14.4025-4032.2002
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发表时间：
2002-07-01
影响因子：
3.2
通讯作者：
Shinnick, TM
中科院分区：
文献类型：
--
作者：
Fisher, MA;Plikaytis, BB;Shinnick, TM
我们使用微阵列和实时逆转录-PCR来分析结核分枝杆菌在体外对低pH的全球转录反应，这可能模拟了被吞噬的分枝杆菌所遇到的环境信号。81个基因差异表达> 1.5倍，包括许多涉及脂肪酸代谢的基因。最高度诱导的基因显示与非核糖体肽合成酶/聚酮酶同源。
We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed > 1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.