PHYSICAL MAPPING AND YAC CONTIG ANALYSIS OF THE REGION SURROUNDING XIST ON THE MOUSE X-CHROMOSOME
PHYSICAL MAPPING AND YAC CONTIG ANALYSIS OF THE REGION SURROUNDING XIST ON THE MOUSE X-CHROMOSOME
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DOI：
10.1006/geno.1993.1108
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发表时间：
1993-03-01
期刊：
影响因子：
4.4
通讯作者：
AVNER, P
中科院分区：
文献类型：
--
作者：
HEARD, E;SIMMLER, MC;AVNER, P
TheXistsequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. We have cloned 550 kb of DNA surrounding the mouseXistsequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map we have established provides a framework for the identification of expressed sequences other thanXistthat may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and we report here the identification of certain CpG-containing restriction sites aroundXistthat have an interesting differential methylation status on the inactive and active X chromosomes.
