Fcγ receptor‐mediated phagocytosis requires tyrosine kinase activity and is ligand independent
Fcγ receptor‐mediated phagocytosis requires tyrosine kinase activity and is ligand independent
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Fcγ受体介导的吞噬作用需要酪氨酸激酶活性并且不依赖于配体
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Allen
中科院分区：
文献类型：
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作者：
M. Hutchinson;P. Harrison;R. Floto;J. Allen
Receptors for the invariant chain of immunoglobulins (FcR) define the cellular response to specific antigens. FcγR recognize IgG and so elicit a variety of effector functions including phagocytosis. We are interested in the structural determinants for FcγR‐mediated phagocytosis, specifically FcγRI(p135) and FcγRIIa isoforms. The low‐affinity receptor, FcγRIIa, is found on macrophages and its cytoplasmic domain contains a tyrosine activation motif which has previously been shown to regulate endocytosis. In contrast, FcγRI has no known signaling motifs, though a functional interaction has recently been demonstrated with the γ chain of the high‐affinity receptor for IgE, FcεRI. This accessory molecule has a cytoplasmic tyrosine activation motif implicated in signal transduction. Here we demonstrate that although FcγRI transiently expressed on COS‐7 cells is able to rosette opsonized SRBC, it cannot phagocytose them. If the cytoplasmic domain of either γ chain or FcγRIIa replaces that of FcγRI in a chimeric receptor, efficient phagocytosis occurs. This particle ingestion is sensitive to the tyrosine kinase inhibitor genistein. Chimeric receptors where the extracellular domain of either FcγRI or FcγRIIa is replaced with that of CD2, a T cell antigen, indicate that FcγR‐mediated phagocytosis is ligand independent. We conclude that phagocytosis is dependent upon close particle apposition, tyrosine kinase activity, and that the process is ligand independent.
免疫球蛋白 G 的高亲和力 Fc 受体 (Fc gamma RI) 的聚集导致其相关的 γ 链磷酸化。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Duchemin,AM;Ernst,LK;Anderson,CL
通讯作者：
Anderson,CL
高亲和力 IgG 受体 (Fc gamma RI) 与高亲和力 IgE 受体 (Fc epsilon RI gamma) 的 γ 亚基之间的物理关联。
DOI：
10.1073/pnas.90.19.8847
发表时间：
1993
影响因子：
11.1
作者：
Scholl,PR;Geha,RS
通讯作者：
Geha,RS
Fc 受体介导的巨噬细胞吞噬作用期间，Fc γ 受体、p72syk 和桩蛋白的 γ 亚基的酪氨酸磷酸化。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Greenberg,S;Chang,P;Silverstein,SC
通讯作者：
Silverstein,SC