Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract
Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract
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DOI：
10.1016/j.foodcont.2007.09.007
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发表时间：
2008-10-01
期刊：
影响因子：
6
通讯作者：
Tawata, Shinkichi
中科院分区：
文献类型：
--
作者：
Ao, Changwei;Li, Anping;Tawata, Shinkichi
以日本冲绳传统药用植物和食品添加剂无花果的提取物为研究对象，对其抗氧化和抑菌活性进行了评价。结果表明，小果树树皮、果实和叶片的甲醇提取物具有良好的抗氧化活性，对革兰氏阳性菌和革兰氏阴性菌也具有抗菌活性。树皮提取物中的乙酸乙酯部分(BE)具有较强的抗氧化和抑菌作用，且总酚含量较高(436 GAE mg/g提取物)。BE在DPPH、ABTS、超氧阴离子自由基清除方法中的EC50值分别为4.83、1.62和63.2mU g/ml。Be对短小芽孢杆菌、蜡状芽孢杆菌、枯草芽孢杆菌、大肠杆菌和无色芽孢杆菌的抑制率分别为18.0、15.5、16.5、16.0和8.0 mm。GC-MS和高效液相色谱分析鉴定出12种酚类化合物。小果皮提取物具有较强的抗氧化性和抗菌活性，这可能与其含有较高的酚类化合物有关。(C)2007爱思唯尔有限公司。保留所有权利。
Antioxidant and antibacterial activities were evaluated by the extracts of Ficus microcarpa, which is one of the traditional medicinal plant and food additives in Okinawa, Japan. Results showed that methanol extracts of bark, fruits and leaves of F microcarpa exhibited excellent antioxidant activities and also possessed antibacterial activity against tested Gram-positive and Gram-negative bacteria. Ethyl acetate fraction of bark extract (BE) exerted strong antioxidant and antibacterial effects and contained high amount of total phenolics (436 GAE mg/g extract). EC50 values of BE were 4.83, 1.62 and 63.2 mu g/ml in DPPH, ABTS(center dot+), superoxide radicals scavenging methods, respectively. Inhibition zones of BE against Bacillus brevis, Bacillus cereus, Bacillus subtilis, Escherichia coli and Achromobacter polymorph were 18.0, 15.5, 16.5, 16.0 and 8.0 mm, respectively. Twelve phenolic compounds were identified in BE fraction by GC-MS and HPLC analyses. The strong antioxidant and antibacterial activities of F microcarpa bark extract may be attributed to its high level of phenolic compounds. (C) 2007 Elsevier Ltd. All rights reserved.