The effect of the entomopathogenic fungus Conidiobolus coronatus on the composition of cuticular and internal lipids of Blatta orientalis females
The effect of the entomopathogenic fungus Conidiobolus coronatus on the composition of cuticular and internal lipids of Blatta orientalis females
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DOI：
10.1111/phen.12133
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发表时间：
2016-06-01
影响因子：
1.5
通讯作者：
Stepnowski, Piotr
中科院分区：
文献类型：
--
作者：
Paszkiewicz, Monika;Golebiowski, Marek;Stepnowski, Piotr
东方蜚蠊雌体表皮和内部脂质的组成。暴露于昆虫病原真菌Conidiobolus coronatus。脂肪酸、正构烷烃、醇、甾醇和甲酯的组成进行了化学表征。虽然与真菌的毒性菌落接触不会引起昆虫死亡，但发现表皮和内部的脂质分布发生了显著变化。orientalis雌性含有24种碳链长度从C6到C22不等的化合物。鉴定的主要表皮脂肪酸有：C16：1、C16：0、C18：1和C18：0。orientalis雌性暴露于C. Coronatus仅含有C8至C20的14种游离脂肪酸。确定的最高浓度为Cl 6：0、C18：2和C18：1。通过气相色谱-质谱分析鉴定出含有25至31个碳原子的正构烷烃的同源系列的存在。在真菌暴露后的昆虫的情况下，表皮脂质中的正构烷烃的含量是对照组的两倍。在表皮脂质中，发现了11种醇，范围从C12：0到C20：0。在对照B的内部脂质中不存在醇。orientalis雌性和来自B的所有提取物中。东方女性真菌暴露后。在分析的样品中，最常见的甾醇是胆固醇。这存在于所有采样昆虫的表皮脂质和内部脂质中。雌性的表皮和内部脂质含有五种脂肪酸甲酯，大小从。C15到C19
The composition of cuticular and internal lipids in females of the cockroach Blatta orientalis L. exposed to the entomopathogenic fungus Conidiobolus coronatus is investigated. The compositions of the fatty acids, n-alkanes, alcohol, sterols and methyl esters in the lipids are chemically characterized. Although contact with virulent colonies of the fungus does not induce insect mortality, significant changes in the lipid profiles, both cuticular and internal, are found. The cuticular extracts of a control group of B. orientalis females contain 24 compounds varying in carbon chain length from C6 to C22. The main cuticular fatty acids identified are: C16:1, C16:0, C18:1 and C18:0 The cuticular lipids of B. orientalis females after exposure to C. coronatus contain only 14 free fatty acids from C8 to C20. The highest concentrations identified are Cl 6:0, C18:2 and C18:1. Analysis by gas chromatography-mass spectrometry identifies the presence of a homologous series of n-alkanes containing from 25 to 31 carbon atoms. In the case of the insects after fungal exposure, the content of the n-alkanes in the cuticular lipid is two-fold higher compared with the controls. Of the cuticular lipids, 11 alcohols are found, ranging from C12:0 to C20:0. There is no presence of alcohols in the internal lipids of the control B. orientalis females and in all of the extracts from the B. orientalis females after fungal exposure. In the samples analyzed, the most common sterol is cholesterol. This is present in the cuticular lipids and the internal lipids of all of the insects sampled. The cuticular and internal lipids of females contain five fatty acid methyl esters, ranging in size from. C15 to C19.