Simple, Low-Cost Molecular Assays for TR34/L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates
Simple, Low-Cost Molecular Assays for TR34/L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates
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DOI：
10.1128/jcm.00408-14
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发表时间：
2014-06-01
影响因子：
9.4
通讯作者：
Meis, Jacques F.
中科院分区：
文献类型：
--
作者：
Ahmad, Suhail;Khan, Ziauddin;Meis, Jacques F.
采用简单、低成本的PCR/PCR-限制性片段长度多态性（RFLP）检测cyp51A启动子和密码子98区域携带TR34/L98H突变的耐三唑烟曲霉菌株。采用40株伊曲康唑敏感株和35株伊曲康唑耐药株进行评价。现在，在资源贫乏的环境中，可以很容易地确定临床/环境烟曲霉分离株中TR34/L98H突变的流行程度。
Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.