Use of a granular bioplastic formulation for carrying conidia of a non-aflatoxigenic strain of Aspergillus flavus
Use of a granular bioplastic formulation for carrying conidia of a non-aflatoxigenic strain of Aspergillus flavus
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DOI：
10.1016/j.biortech.2009.03.010
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发表时间：
2009-09-01
影响因子：
11.4
通讯作者：
Wilkinson, Jeffery R.
中科院分区：
文献类型：
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作者：
Accinelli, Cesare;Sacca, M. Ludovica;Wilkinson, Jeffery R.
以往的研究表明，用非黄曲霉菌株NRRL-30797预接种小麦籽粒可以减少玉米中的黄曲霉毒素污染。为了便于该生防分离物的田间应用，进行了一系列的室内试验，研究了用新型生物塑料制剂Mater-BI(R)代替小麦籽粒作为载体基质配制该真菌的可靠性和有效性。用NRRL 30797的分生孢子悬浮液接种Mater-BiR(R)颗粒，以获得大约log7分生孢子/颗粒的最终细胞密度。在微生物活性土壤和灭菌土壤中，加入单一Mater-BI(R)颗粒的20克土壤样品培养60天，每克土壤中黄曲霉的繁殖率分别为对数4.2-5.3。颗粒数的增加对生防真菌侵染土壤的程度没有影响。除了保持土壤样品的快速营养生长和定植外，生物塑料制剂具有高度的稳定性，表明MATER-BI(R)是黄曲霉NRRL 30797生防应用的合适替代品。(C)2009爱思唯尔有限公司。保留所有权利。
Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of this biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi (R) to serve as a carrier matrix to formulate this fungus. Mater-Bi (R) granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of approximately log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi (R) granule for 60-days resulted in log 4.2-5.3 propagules of A.flavus/g soil in microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi (R) is a suitable substitute for biocontrol applications of A. flavus NRRL 30797. (C) 2009 Elsevier Ltd. All rights reserved.