Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.
Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.
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DOI：
10.1126/science.1189435
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发表时间：
2010-10-08
期刊：
影响因子：
--
通讯作者：
Higgins JM
中科院分区：
文献类型：
--
作者：
Wang F;Dai J;Daum JR;Niedzialkowska E;Banerjee B;Stukenberg PT;Gorbsky GJ;Higgins JM
Aurora-B是染色体乘客复合物（CPC）的一个组成部分，在染色体分离和胞质分裂过程中正确的纺锤体-动粒附着所需。然而，将CPC募集到着丝粒的染色质因子尚不清楚。在这里，我们表明，磷酸化组蛋白-H3 Thr-3（H3 T3 ph）的Haspin是必要的CPC在着丝粒的积累，和CPC亚基生存素直接结合到H3 T3 ph。非结合性Survivin-D 70 A/D 71 A突变体不支持着丝粒CPC浓度，并且Haspin缺失和Survivin-D 70 A/D 71 A突变都减少了MCAK的着丝粒定位和紫杉醇中的有丝分裂检查点信号传导。Survivin-D 70 A/D 71 A突变和H3 T3 ph特异性抗体的显微注射都损害了着丝粒Aurora-B功能，但不能阻止胞质分裂。因此，Haspin产生的H3 T3 ph将CPC定位在着丝粒上，以在有丝分裂期间调节Aurora-B的选定靶标。
Aurora-B is a component of the Chromosomal Passenger Complex (CPC) required for correct spindle-kinetochore attachments during chromosome segregation and for cytokinesis. The chromatin factors that recruit the CPC to centromeres are unknown, however. Here we show that phosphorylation of Histone-H3 Thr-3 (H3T3ph) by Haspin is necessary for CPC accumulation at centromeres, and that the CPC subunit Survivin binds directly to H3T3ph. A non-binding Survivin-D70A/D71A mutant does not support centromeric CPC concentration and both Haspin depletion and Survivin-D70A/D71A mutation diminish centromere localization of MCAK and mitotic checkpoint signaling in taxol. Survivin-D70A/D71A mutation and microinjection of H3T3ph-specific antibody both compromise centromeric Aurora-B functions but do not prevent cytokinesis. Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora-B during mitosis.
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影响因子：
4.8
作者：
Song, ZY;Yao, XB;Wu, M
通讯作者：
Wu, M
影响因子：
7.8
作者：
Yue, Zuojun;Carvalho, Ana;Xu, Zhenjie;Yuan, Xuemei;Cardinale, Stefano;Ribeiro, Susana;Lai, Fan;Ogawa, Hiromi;Gudmundsdottir, Elisabet;Gassmann, Reto;Morrison, Ciaran G.;Ruchaud, Sandrine;Earnshaw, William C.
通讯作者：
Earnshaw, William C.
影响因子：
9.8
作者：
McGuinness BE;Hirota T;Kudo NR;Peters JM;Nasmyth K
通讯作者：
Nasmyth K
DOI：
10.1083/jcb.200404001
发表时间：
2004-07-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gassmann R;Carvalho A;Henzing AJ;Ruchaud S;Hudson DF;Honda R;Nigg EA;Gerloff DL;Earnshaw WC
通讯作者：
Earnshaw WC
影响因子：
56.9
作者：
Rosasco-Nitcher, Sara E.;Lan, Weijie;Stukenberg, P. Todd
通讯作者：
Stukenberg, P. Todd