Redistribution of adhering junctions in human endometrial epithelial cells during the implantation window of the menstrual cycle
Redistribution of adhering junctions in human endometrial epithelial cells during the implantation window of the menstrual cycle
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DOI：
10.1007/s00418-012-0929-0
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发表时间：
2012-06-01
影响因子：
2.3
通讯作者：
Classen-Linke, Irmgard
中科院分区：
文献类型：
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作者：
Buck, Volker U.;Windoffer, Reinhard;Classen-Linke, Irmgard
人类子宫上皮的特点是具有显着的可塑性和分化的周期性变化，这些变化由卵巢类固醇激素控制，以优化胚胎植入的条件。为了了解细胞间粘附是否以及如何受到影响，我们对整个月经周期中连接蛋白的定位进行了研究。通过免疫荧光检查了不同周期阶段的 36 份人类子宫内膜标本的表达模式。抗桥粒蛋白桥粒斑蛋白 1/2 (Dp 1/2) 和桥粒芯糖蛋白 2 (Dsg 2)、粘附连接蛋白 E-钙粘蛋白和 β-连环蛋白以及常见连接接头蛋白斑珠蛋白的抗体在增殖期直至黄体期早期（第 20 天）显示出强烈的根尖下染色。然而，在黄体期中后期，这些连接蛋白在整个侧质膜上重新分布。相反，紧密连接蛋白（ZO-1、claudin 4）在整个月经周期中保持在其特征性的根尖下位置。通过实时RT-PCR获得的Dp 1/2、E-钙粘蛋白和ZO-1的mRNA水平在月经周期期间没有显着变化。观察到的桥粒和粘附连接的重新分布与所谓的人类子宫内膜植入窗口的开始一致。我们认为这种变化是由卵巢类固醇控制的，并为子宫内膜成功滋养层侵袭做好准备。
The human uterine epithelium is characterised by remarkable plasticity with cyclic changes in differentiation that are controlled by ovarian steroid hormones to optimise conditions for embryo implantation. To understand whether and how cell-cell adhesion is affected, the localisation of junction proteins was studied throughout the menstrual cycle. Expression patterns were examined by immunofluorescence in 36 human endometrial specimens of different cycle stages. Antibodies against the desmosomal proteins desmoplakin 1/2 (Dp 1/2) and desmoglein 2 (Dsg 2), the adherens junction proteins E-cadherin and beta-catenin and also the common junctional linker protein plakoglobin showed a strong subapical staining during the proliferative phase until the early luteal phase (day 20). In the mid- to late luteal phase, however, these junctional proteins redistributed over the entire lateral plasma membranes. In contrast, tight junction proteins (ZO-1, claudin 4) remained at their characteristic subapical position throughout the menstrual cycle. mRNA levels of Dp 1/2, E-cadherin and ZO-1 obtained by real time RT-PCR were not significantly changed during the menstrual cycle. The observed redistribution of desmosomes and adherens junctions coincides with the onset of the so called implantation window of human endometrium. We propose that this change is controlled by ovarian steroids and prepares the endometrium for successful trophoblast invasion.