An animal model of nociceptive peripheral neuropathy following repeated cisplatin injections
An animal model of nociceptive peripheral neuropathy following repeated cisplatin injections
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DOI：
10.1016/s0014-4886(03)00003-7
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发表时间：
2003-07-01
影响因子：
5.3
通讯作者：
Coudore, F
中科院分区：
文献类型：
--
作者：
Authier, N;Gillet, JP;Coudore, F
我们报告的运动和感觉行为的评估，使用电生理和组织学的方法，在大鼠模型顺铂周围神经病变。每周腹膜内注射顺铂1次（3 mg/ kg）、2次（2 mg/kg）或3次（1 mg/kg），累积剂量为15或20 mg/kg。关于伤害性体征，我们观察到3 mg/kg剂量的机械和热（冷刺激）痛觉过敏和与轻微运动障碍相关的异常性疼痛。顺铂（3 mg/kg）治疗组的外周神经传导速度降低。此外，组织学方法显示，大的轴突比小的更频繁地受到影响，无髓鞘轴突不受影响。然而，即使在最严重的情况下，髓鞘仍然在正常范围内。这种伤害性神经病变的动物模型将适合于研究神经病理性疼痛的病理生理机制和测试潜在的神经保护剂。(C)2003 Elsevier Science（美国）。版权所有copyright ©
We report the assessment of motor and sensory behaviors using an electrophysiologic and an histologic approach, in a rat model of cisplatin peripheral neuropathy. Cisplatin was injected intraperitoneally one (3 mg/ kg), two (2 mg/kg), or three (1 mg/kg) times a week up to a cumulative dose of 15 or 20 mg/kg. With regard to nociceptive signs, we observed mechanical and thermal (cold stimuli) hyperalgesia and allodynia associated with minor motor disorders for the 3 mg/kg dose. Peripheral nerve conduction velocities were decreased in the cisplatin-(3 mg/kg) treated group. In addition, the histologic approach revealed that large axons were more frequently affected than the small ones, and nonmyelinated axons were unaffected. However, even in the most severe cases, myelin sheaths remained within normal limits. This animal model of nociceptive neuropathy would be suitable to study the pathophysiologic mechanisms of neuropathic pain and to test potential neuroprotective agents. (C) 2003 Elsevier Science (USA). All rights preserved.