Transductional and transcriptional targeting of cancer cells using genetically engineered viral vectors
Transductional and transcriptional targeting of cancer cells using genetically engineered viral vectors
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DOI：
10.1016/j.canlet.2003.07.003
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发表时间：
2003-11-25
期刊：
影响因子：
9.7
通讯作者：
Baker, AH
中科院分区：
文献类型：
--
作者：
Nicklin, SA;Dishart, KL;Baker, AH
基因递送载体，包括腺病毒（Ad）和腺相关病毒（AAV），由于在其他组织（包括肝脏）上具有高水平的低水平病毒受体，因此对于癌症是低效的和非选择性的。我们测试了具有插入病毒衣壳中的SIGYPLP靶向肽的Ad和AAV在一组癌细胞中的转导。12个细胞系中的6个（C8161、PC-3、G-CCM、MKN-45、LnCAP和A549）被转导，与天然病毒嗜性无关。此外，候选癌症基因治疗启动子FLT-1在这六种细胞系中的三种中具有活性。这提供了双重靶向选定癌细胞的可能性。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
Gene delivery vectors, including adenovirus (Ad) and adeno-associated virus (AAV), are inefficient and non-selective for cancer due to low levels of viral receptors with high levels on other tissues, including liver. We tested Ads and AAVs with the SIGYPLP-targeting peptide inserted into virus capsids for transduction in a panel of cancer cells. Six of twelve lines (C8161, PC-3, G-CCM, MKN-45, LnCAP and A549) were transduced, independently of native viral tropism. Furthermore the candidate cancer gene therapy promoter FLT-1 was active in three of these six cell lines. This offers the potential for dual targeting of selected cancer cells. (C) 2003 Elsevier Ireland Ltd. All rights reserved.