Direct Production of L-Tagatose from L-Psicose by Enterobacter aerogenes 230S
Direct Production of L-Tagatose from L-Psicose by Enterobacter aerogenes 230S
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DOI：
10.1263/jbb.106.473
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发表时间：
2008-11-01
影响因子：
2.8
通讯作者：
Izumori, Ken
中科院分区：
文献类型：
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作者：
Rao, Devendar;Gullapalli, Pushpakiran;Izumori, Ken
L-塔格糖是由 L-阿洛酮糖直接生产的，方法是将相同的生物质悬浮液进行微生物还原，然后使用新分离的细菌产气肠杆菌 230S 进行氧化。经过各种优化后，观察到在木糖醇上生长的细胞具有最佳的转化潜力。此外，在多元醇如甘油、D-山梨糖醇、核糖醇、L-阿拉伯糖醇、D-甘露糖醇和木糖醇存在下，产气肠球菌230S以更快的速率将L-阿洛酮糖转化为L-塔格糖。在底物浓度为 5% 的甘油存在下，L-阿洛酮糖向 L-塔格糖的转化率高于 60%。通过 HPLC 分析、C-13-NMR 光谱和旋光度证实了结晶 L-塔格糖的身份。
L-Tagatose was produced directly from L-psicose by subjecting the same biomass suspension to microbial reduction followed by oxidation using a newly isolated bacteria Enterobacter aerogenes 230S. After various optimizations, it was observed that cells grown on xylitol have the best conversion potential. Moreover, E. aerogenes 230S converted L-psicose to L-tagatose at a faster rate in the presence of polyols such as glycerol, D-sorbitol, ribitol, L-arabitol, D-mannitol and xylitol. At 5% substrate concentration, the conversion ratio of L-psicose to L-tagatose was above 60% in the presence of glycerol. Identity of crystalline L-tagatose was confirmed by HPLC analysis, C-13-NMR spectra, and optical rotation.