Reaction of caa3-type terminal cytochrome oxidase from the thermophilic bacterium PS3 with oxygen and carbon monoxide at low temperatures.
Reaction of caa3-type terminal cytochrome oxidase from the thermophilic bacterium PS3 with oxygen and carbon monoxide at low temperatures.
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嗜热细菌 PS3 的 caa3 型末端细胞色素氧化酶在低温下与氧气和一氧化碳发生反应。
DOI：
10.1042/bj2210529
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Chance,B
中科院分区：
文献类型：
--
作者：
Sone,N;Naqui,A;Kumar,C;Chance,B
研究了低温下O2和CO与高温通气条件下嗜热菌PS3的Caa 3型末端细胞色素氧化酶（EC 1.9.3.1）的反应。在研究的温度范围内（−50摄氏度至−80摄氏度），CO复合遵循一级动力学，活化能为29.3 kJ/mol（7.0 kcal/mol）。在-113 ° C存在O2的情况下，光解的还原形式结合O2以形成类似于化合物A的“氧基”中间体。在较高温度（-97 ℃）下，由于电子从酶转移到配体O2，形成了另一种类似于化合物B的中间体。
Reaction of O2 and CO with a caa3-type terminal cytochrome oxidase (EC 1.9.3.1) from the thermophilic bacterium PS3 grown with high aeration was studied at low temperatures. The CO recombination at the temperature range studied (−50 degrees C to −80 degrees C) followed first-order kinetics with an activation energy of 29.3 kJ/mol (7.0 kcal/mol). In the presence of O2 at −113 degrees C the photolysed reduced form binds O2 to form an ‘oxy’ intermediate similar to Compound A. At a higher temperature (-97 degrees C) another intermediate, similar to Compound B, is formed as a result of electron transfer from the enzyme to the liganded O2.