Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy.
Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy.
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DOI：
10.1007/978-1-59259-993-6_3
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发表时间：
2006
影响因子：
--
通讯作者：
M. Roe;J. Fiekers;L. Philipson;V. Bindokas
中科院分区：
文献类型：
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作者：
M. Roe;J. Fiekers;L. Philipson;V. Bindokas
钙离子（Ca2+）是细胞信号转导的重要组成部分。细胞质和细胞器内Ca 2+浓度的动态变化受到严格控制，并调节各种生物活动，包括受精、细胞分裂、基因表达、细胞代谢、蛋白质生物合成、分泌、肌肉收缩、细胞间通讯和细胞死亡。细胞内[Ca2+]的测量对于理解Ca2+的作用和定义任何细胞过程中的潜在调节机制至关重要。广泛的合成和生物合成荧光Ca2+传感器可用于亚细胞时空[Ca2+]梯度的可视化和定量。本章介绍了宽场数字化视频荧光显微荧光和共聚焦显微镜定量图像钙离子在细胞中具有高的时间和空间分辨率的应用。
Calcium (Ca2+) is a fundamentally important component of cellular signal transduction. Dynamic changes in the concentration of Ca2+([Ca2+]) in the cytoplasm and within organelles are tightly controlled and regulate a diverse array of biological activities, including fertilization, cell division, gene expression, cellular metabolism, protein biosynthesis, secretion, muscle contraction, intercellular communication, and cell death. Measurement of intracellular [Ca2+] is essential to understanding the role of Ca2+and for defining the underlying regulatory mechanisms in any cellular process. A broad range of synthetic and biosynthetic fluorescent Ca2+sensors are available that enable the visualization and quantification of subcellular spatio-temporal [Ca2+] gradients. This chapter describes the application of wide-field digitized video fluorescence microfluorometry and confocal microscopy to quantitatively image Ca2+in cells with high temporal and spatial resolution.