Structural analysis of gubernaculum testis in cryptorchid patients submitted to treatment with human chorionic gonadotrophin
Structural analysis of gubernaculum testis in cryptorchid patients submitted to treatment with human chorionic gonadotrophin
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DOI：
10.1590/s1677-55382007000200014
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发表时间：
2007-04-01
影响因子：
3.7
通讯作者：
Sampaio, Francisco J. B.
中科院分区：
文献类型：
--
作者：
El Zoghbi, Charbel S.;Favorito, Luciano A.;Sampaio, Francisco J. B.
目的：观察人绒毛膜促性腺激素（hCG）治疗隐睾患者睾丸管弹性系统纤维、胶原蛋白和横纹肌细胞的组织学和体视学变化。材料与方法：从12例隐睾患者的肺组织中提取肺组织标本。患者年龄1 ~ 3岁（平均1.8岁）。12例患者中，6例接受hCG治疗。样品用马森三色染色，以突出肌肉纤维和胶原蛋白，并用威格特的间瘤素-紫红染色，以突出弹性系统纤维。用立体学方法测定了这些组分的体积密度。结果：hCG治疗组胶原体积密度升高，为85.62% ~ 94.48%，未接受hCG治疗组胶原体积密度升高，为52.12% ~ 89.46% （p=0.0052）。hCG治疗组弹性系统纤维体积密度较高，为9.62% ~ 19.62%，未接受hCG治疗组弹性系统纤维体积密度为10.38% ~ 12.38% （p=0.0030）。接受hCG治疗的患者横纹肌纤维体积密度范围为4.76% ~ 39.16%，未接受hCG治疗的患者横纹肌纤维体积密度范围为3.24% ~ 11.14% （p=0.0052）。结论：当接受hCG治疗时，地方成分发生显著变化。接受hCG治疗且睾丸未完全迁移的患者弹性肌纤维和横纹肌纤维浓度增加，胶原体积密度降低。
OBJECTIVES: To make a histological and stereological analysis of gubernaculum testis elastic system fibers, collagen and striated muscle cells in patients with cryptorchidism treated with human chorionic gonadotrophin (hCG).MATERIALS AND METHODS: Gubernacula tissue samples were obtained from 12 patients with cryptorchidism. Patients' ages ranged from 1 to 3 years (mean 1.8). Of the 12 patients, 6 were treated with hCG. The samples were stained with Masson's trichrome to highlight muscle fibers and collagen, and Weigert's resorcin-fuchsin to highlight the elastic system fibers. The volumetric density of these components was determined by stereological methods.RESULTS: The volumetric density of collagen was increased in patients treated with hCG, ranging from 85.62% to 94.48%, while in patients not submitted to hCG treatment the volume density ranged from 52.12% to 89.46% (p=0.0052). The volumetric density of the elastic system fibers was higher in patients treated with hCG, ranging from 9.62% to 19.62%, while patients not submitted to hCG treatment the volume density of elastic system fibers was between 10.38% and 12.38% (p=0.0030). The volume density of striated muscle fibers in patients treated with hCG ranged from 4.76% to 39.16%, while and in patients not treated hCG it ranged from 3.24% to 11.14% (p=0.0052).CONCLUSION: Gubernacular components alter significantly when submitted to treatment with hCG. Patients who underwent hCG treatment and had no complete testicular migration had an increase in the concentration of elastic and striated muscle fibers and a decrease in the volumetric density of collagen.