Inhibition of hydrogen-yielding dark fermentation by ascomycetous yeasts
Inhibition of hydrogen-yielding dark fermentation by ascomycetous yeasts
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DOI：
10.1016/j.ijhydene.2018.05.004
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发表时间：
2018-06-14
影响因子：
7.2
通讯作者：
Sikora, Anna
中科院分区：
文献类型：
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作者：
Detman, Anna;Chojnacka, Aleksandra;Sikora, Anna
在生物反应器中进行的产氢发酵是一种替代的制氢方法。然而，在厌氧消化的产酸步骤中，不利的过程会严重抑制生物制氢。在这里，子囊菌酵母被确定为抑制通过发酵生产生物氢的主要因素。产氢生物反应器的性能变化，包括代谢转变，发酵产物的定量变化，pH值降低，微生物群落的不稳定性，从而在生物氢产量急剧下降后，观察到酵母感染。从产氢生物反应器中分离出来自假丝酵母属、哈萨克斯坦酵母属和地霉属的子囊酵母。分泌到生长培养基中的酵母代谢产物显示出抗菌活性。我们的研究表明，酵母菌对产氢微生物群落的感染是使用暗发酵作为生物制氢的替代方法的严重障碍之一。这也解释了为什么氢发酵的研究仍然局限于实验室或中试规模的系统。(C)2018作者（S）由爱思唯尔有限公司代表氢能出版有限责任公司出版。
Hydrogen-yielding fermentation conducted in bioreactors is an alternative method of hydrogen production. However, unfavourable processes can seriously inhibit bio-hydrogen generation during the acidogenic step of anaerobic digestion. Here, ascomycetous yeasts were identified as a major factor inhibiting the production of bio-hydrogen by fermentation. Changes in the performance of hydrogen-producing bioreactors including metabolic shift, quantitative changes in the fermentation products, decreased pH, instability of the microbial community and consequently a dramatic drop in bio-hydrogen yield were observed following yeast infection. Ascomycetous yeasts from the genera Candida, Kazachstania and Geotrichum were isolated from hydrogen-producing bioreactors. Yeast metabolites secreted into the growth medium showed antibacterial activity. Our studies indicate that yeast infection of hydrogen -producing microbial communities is one of the serious obstacles to use dark fermentation as an alternative method of bio-hydrogen production. It also explains why studies on hydrogen fermentation are still limited to the laboratory or pilot-scale systems. (C) 2018 The Author(s). Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC.