Calycosin and Genistein Induce Apoptosis by Inactivation of HOTAIR/p-Akt Signaling Pathway in Human Breast Cancer MCF-7 Cells
Calycosin and Genistein Induce Apoptosis by Inactivation of HOTAIR/p-Akt Signaling Pathway in Human Breast Cancer MCF-7 Cells
复制标题
毛蕊异黄酮和金雀花素通过灭活人乳腺癌 MCF-7 细胞中的 HOTAIR/p-Akt 信号通路诱导细胞凋亡
DOI：
10.1159/000369732
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Huang, Zhaoquan
中科院分区：
文献类型：
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作者：
Chen, Jian;Lin, Changrong;Huang, Zhaoquan
背景：毛蕊异黄酮和染料木素是毛蕊异黄酮的两个主要成分。以前，我们报道了这些化合物在乳腺癌细胞系MCF-7和T47 D中显示出抗肿瘤活性。在本研究中，我们探讨了毛蕊异黄酮和染料木黄酮的作用机制，以及它们各自作为临床治疗乳腺癌的潜在疗法的功效。方法：用毛蕊异黄酮或染料木黄酮处理MCF-7细胞。使用CCK 8测定和Hoechst 33258测量细胞增殖和凋亡。Western blotting检测磷酸化Akt蛋白的表达水平。通过实时PCR定量HOTAIR的表达水平。结果如下：毛蕊异黄酮和染料木黄酮均能抑制MCF-7乳腺癌细胞的增殖并诱导其凋亡，尤其是毛蕊异黄酮处理后。用毛蕊异黄酮或染料木黄酮处理MCF-7细胞导致Akt磷酸化降低，其下游靶点HOTAIR的表达降低。结论：毛蕊异黄酮通过调节Akt信号通路和HOTAIR的表达，比染料木素更有效地抑制乳腺癌的生长。
Background: Calycosin and genistein are the two main components of isoflavones. Previously, we reported that these compounds display antitumor activities in the breast cancer cell lines MCF-7 and T47D. In the present study, we investigated the mechanism of action of calycosin and genistein, and their respective efficacies as potential therapies for the treatment of breast carcinoma in the clinic. Methods: MCF-7 cells were treated with calycosin or genistein. Cell proliferation and apoptosis were measured using CCK8 assay and Hoechst 33258. The expression level of phosphorylated Akt protein was determined by western blotting. Expression level of HOTAIR was quantified by real-time PCR. Results: Both calycosin and genistein inhibited proliferation and induced apoptosis in MCF-7 breast cancer cells, especially after treatment with calycosin. Treatment of MCF-7 cells with calycosin or genistein resulted in decreased phosphorylation of Akt, and decreased expression of its downstream target, HOTAIR. Conclusion: Calycosin is more effective in inhibiting breast cancer growth in comparison with genistein, through its regulation of Akt signaling pathways and HOTAIR expression.