Molecular analysis of Ac transposition and DNA replication.
Molecular analysis of Ac transposition and DNA replication.
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Ac 转座和 DNA 复制的分子分析。
DOI：
10.1093/genetics/130.3.665
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Dellaporta,SL
中科院分区：
文献类型：
--
作者：
Chen,J;Greenblatt,IM;Dellaporta,SL
与玉米P基因座上的移动的元件激活子（Ac）转座相关的分子事件已在双生扇区的子代谱系中进行了研究。遗传学和分子生物学分析表明，供体Ac仅从这些谱系中的两个子染色体之一切除。子代染色体上靶位点的克隆和序列分析表明，Ac插入可以发生在DNA复制完成之前或之后。从复制供体位点到未复制和复制靶位点的转座意味着Ac的大多数转座发生在细胞周期的S期期间或之后不久。
Molecular events associated with transposition of the mobile element Activator (Ac) from the P locus of maize have been examined in daughter lineages of twinned sectors. Genetic and molecular analyses indicate that the donor Ac has excised from only one of the two daughter chromosomes in these lineages. Cloning and sequence analyses of target sites on daughter chromosomes indicate that Ac insertion can occur either before or after the completion of DNA replication. Transpositions from a replicated donor site to both unreplicated and replicated target sites imply that most transpositions of Ac occur during or shortly after the S phase of the cell cycle.