Comparison of randomly cloned and whole genomic DNA probes for the detection of Porphyromonas gingivalis and Bacteroides forsythus.
Comparison of randomly cloned and whole genomic DNA probes for the detection of Porphyromonas gingivalis and Bacteroides forsythus.
复制标题
用于检测牙龈卟啉单胞菌和福赛拟杆菌的随机克隆探针和全基因组 DNA 探针的比较。
DOI：
10.1111/j.1600-0765.1996.tb00460.x
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发表时间：
1996
影响因子：
3.5
通讯作者：
Listgarten,MA
中科院分区：
文献类型：
--
作者：
Wong,M;DiRienzo,JM;Lai,CH;Listgarten,MA
用地高辛标记两种苛求牙周病原体牙龈卟啉单胞菌和拟杆菌的全基因组和随机克隆DNA探针，并通过比色法进行检测。比较了全基因组探针和克隆探针的特异性和灵敏度。与全基因组探针相比，克隆探针具有高度特异性。与类杆菌属、二氧化碳噬细胞菌的交叉反应性显著。和普雷沃特利亚斯。用全基因组探针观察。克隆探针的灵敏度低于全基因组探针，需要至少106个靶标。细胞或在杂交过程中检测的最小10 ng靶DNA。尽管全基因组探针的灵敏度提高了10倍，但与密切相关物种的交叉杂交限制了它们在龈下菌斑样本中识别靶细菌的可靠性。
Whole genomic and randomly‐cloned DNA probes for two fastidious periodontal pathogens,Porphyromonas gingivalisandBacteroides forsythuswere labeled with digoxigenin and detected by a colorimetric method. The specificity and sensitivity of the whole genomic and cloned probes were compared. The cloned probes were highly specific compared to the whole genomic probes. A significant degree of cross‐reactivity withBacteroidesspecies.Capnocytophagasp. andPrevotellasp. was observed with the whole genomic probes. The cloned probes were less sensitive than the whole genomic probes and required at least 106 target. cells or a mininium of 10 ng of target DNA to be detected during hybridization. Although a ten‐fold increase in sensitivity was obtained with the whole genomic probes, cross‐hybridization to closely related species limits their reliability in identifying target bacteria in subgingival plaque samples.