Remote eradication of biofilm on titanium implant via near-infrared light triggered photothermal/photodynamic therapy strategy
Remote eradication of biofilm on titanium implant via near-infrared light triggered photothermal/photodynamic therapy strategy
复制标题
通过近红外光触发光热/光动力治疗策略远程消除钛种植体上的生物膜
DOI：
10.1016/j.biomaterials.2019.119479
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发表时间：
2019
期刊：
影响因子：
14
通讯作者：
Cai Kaiyong
中科院分区：
文献类型：
--
作者：
Yuan Zhang;Tao Bailong;He Ye;Mu Caiyun;Liu Genhua;Zhang Jixi;Liao Qiang;Liu Peng;Cai Kaiyong
生物膜的形成是骨植入物相关感染治疗的主要挑战，导致对免疫系统和抗生素的耐受。然而，对抗植入物上已形成的生物膜的智能非手术或非侵入性治疗方法报道较少。本文首次报道了由介孔聚多巴胺纳米颗粒（MPDA）组成的治疗系统，以对抗生物膜。我们开发了一种协同光热/光动力治疗（PTT/PDT）策略，旨在根除钛（Ti）种植体上的生物膜，该策略与光敏剂吲哚菁绿色（ICG）通过π-π堆积负载的MPDA相结合。具体地，MPDA用RGD肽官能化以赋予修饰的Ti样品（Ti-M/I/RGD）良好的细胞相容性。更重要的是，Ti-M/I/RGD植入物显著抑制金黄色葡萄球菌（S.金黄色葡萄球菌生物膜的活体近红外（NIR）检测效率为95.4%。生物膜清除后，种植体在成骨和骨整合方面仍表现出良好的性能。总体而言，本研究为开发用于潜在骨科应用的抗菌钛植入物提供了PTT/PDT策略。
Biofilm formation is a main challenge in treatment of bone-implant-associated infections, resulting in tolerance to immune system and antibiotics. However, smart non-surgical or non-invasive treatment methods of combating established biofilm on an implant have been less reported. Herein, a therapeutic system consisting of mesoporous polydopamine nanoparticles (MPDA) to combat biofilm is reported for the first time. We develop a synergistic photothermal/photodynamic therapy (PTT/PDT) strategy aiming for biofilms eradication on titanium (Ti) implant, which is integrated with MPDA loading with photosensitizer Indocyanine Green (ICG) by π-π stacking. Specifically, MPDA is functionalized with RGD peptide to endow the modified Ti sample (Ti-M/I/RGD) with good cytocompatibility. More importantly, Ti-M/I/RGD implant remarkably killsStaphylococcus aureus(S. aureus) biofilm with an efficiency of 95.4%in vivoupon near infrared (NIR). After biofilm eradication, implant still displays great performance regarding osteogenesis and osseointegration. Overall, this study provides a PTT/PDT strtategy for the development of antibacterial Ti implants for potential orthpediac application.