Functional cloning of genes encoding dsRNA binding proteins.
Functional cloning of genes encoding dsRNA binding proteins.
复制标题
编码 dsRNA 结合蛋白的基因的功能克隆。
DOI：
10.1016/s1046-2023(03)00054-9
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Tolias,PeterP
中科院分区：
文献类型：
--
作者：
Ramanathan,Y;Song,Liting;Mathews,MichaelB;Tolias,PeterP
超过12个人类基因编码特异性结合双链RNA的蛋白质。这些蛋白质参与了几个重要的细胞过程，如转录激活、抑制翻译起始、RNA编辑、mRNA定位、信号转导和转录后基因沉默（PTGS或RNAi）。最近发现PTGS或RNAi是dsRNA介导的途径，进一步增加了对dsRNA结合蛋白的研究。能够克隆编码dsRNA结合蛋白的基因的方法将极大地促进这些分子的鉴定和研究。在这里，我们描述了一种从表达文库中分离这些基因的方法，使用放射性标记的聚（I）：聚（C）作为结合底物。
Over a dozen human genes code for proteins that specifically bind to double-stranded RNA. These proteins have been implicated in several important cellular processes such as transcriptional activation, inhibition of translational initiation, RNA editing, mRNA localization, signal transduction, and posttranscriptional gene silencing (PTGS or RNAi). The recent discovery that PTGS or RNAi is a dsRNA-mediated pathway has further added to the study of dsRNA binding proteins. A method that enables the cloning of genes encoding dsRNA binding proteins would greatly facilitate the identification and study of these molecules. Here we describe a method for isolating such genes from an expression library using radiolabeled poly(I):poly(C) as a binding substrate.