Oscillospira and related bacteria - From metagenomic species to metabolic features
Oscillospira and related bacteria - From metagenomic species to metabolic features
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DOI：
10.1111/1462-2920.13658
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发表时间：
2017-03-01
影响因子：
5.1
通讯作者：
Nielsen, Henrik Bjorn
中科院分区：
文献类型：
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作者：
Gophna, Uri;Konikoff, Tom;Nielsen, Henrik Bjorn
颤螺旋菌属是一种研究不足的厌氧细菌属，来自梭菌簇IV，自从第一次被观察到以来，它已经抵抗了超过世纪的培养。近年来，它的16S rRNA基因在几项人类肠道微生物群研究中被鉴定出来，它通常与有趣的特征有关，特别是瘦。然而，很少有人知道它的代谢或生理。在这里，我们使用来自人类肠道的霰弹枪宏基因组数据的几乎完整的基因组来分析颤螺旋菌和相关细菌。我们使用序列相似性，基因邻域信息和手动代谢途径策展来破译这个有趣的细菌属的关键代谢特征。我们推断，颤螺旋菌属物种是丁酸生产者，并且其中至少一些具有利用葡萄糖醛酸的能力，葡萄糖醛酸是一种常见的动物源性糖，其由人类宿主产生并由该宿主在富含动物产品的饮食中消耗。这些发现可以帮助解释粪便中颤螺旋体水平的饮食相关个体间差异，以及观察到该属的存在在涉及炎症的疾病中减少。
Oscillospira is an under-studied anaerobic bacterial genus from Clostridial cluster IV that has resisted cultivation for over a century since the first time it was observed. In recent years its 16S rRNA gene was identified in several human gut microbiota studies where it was often associated with interesting traits, especially leanness. However, very little is known about its metabolism or physiology. Here we used nearly complete genomes derived from shot-gun metagenomic data from the human gut to analyze Oscillospira and related bacteria. We used sequence similarity, gene neighbourhood information and manual metabolic pathway curation to decipher key metabolic features of this intriguing bacterial genus. We infer that Oscillospira species are butyrate producers, and at least some of them have the ability to utilize glucuronate, a common animal-derived sugar that is both produced by the human host and consumed by that host in diets rich in animal products. These findings could help explain diet-related inter-individual variation in faecal Oscillospira levels as well as the observation that the presence of this genus is reduced in diseases that involve inflammation.