Direct selection of human genomic loci by microarray hybridization
Direct selection of human genomic loci by microarray hybridization
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DOI：
10.1038/nmeth1111
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发表时间：
2007-11-01
期刊：
影响因子：
48
通讯作者：
Gibbs, Richard A.
中科院分区：
文献类型：
--
作者：
Albert, Thomas J.;Molla, Michael N.;Gibbs, Richard A.
我们应用高密度微阵列富集人类基因组中的特定序列进行高通量测序。在捕获6，726个约500个碱基的“外显子”片段和大小范围为200 kb至5 Mb的“基因座特异性”区域后，随后在454 Life Sciences FLX测序仪上测序，大多数序列读数代表选择靶。这些直接选择方法取代了用于基因组区域的大规模分析的多重PCR。
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.