Antiperoxidant pretreatment and iron‐induced epileptiforom discharges in the rat
Antiperoxidant pretreatment and iron‐induced epileptiforom discharges in the rat
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DOI：
10.1212/wnl.31.1.63
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发表时间：
1981-01
期刊：
影响因子：
9.9
通讯作者：
L. Willmore;J. J. Rubin-J.
中科院分区：
文献类型：
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作者：
L. Willmore;J. J. Rubin-J.
铁引起超氧自由基的形成，导致组织膜成分的过氧化。脑内含铁血黄素沉积常伴随创伤后慢性癫痫发作。我们将铁盐（全血中的主要金属离子）的水溶液注射到大鼠同皮质。连续脑电图记录显示，94%的未治疗动物出现癫痫样放电。用α-生育酚和2 ppm硒预处理可防止72%的动物出现铁诱导的癫痫样活动。用Nissl、苏木精和伊红以及普鲁士蓝染色的连续切片的组织学评估显示未处理动物中的空洞化、神经元固缩和丢失以及星形胶质细胞增生。在用抗氧化剂治疗的动物中，铁注射部位仅包含一个神经元固缩区域。抗氧化剂在防止铁诱导的空洞，神经胶质增生和癫痫样放电的发展的功效表明，过氧化损伤可能是重要的实验性癫痫的发展引起的氯化亚铁等皮质注射。
Iron causes formation of superoxide radicals resulting in peroxidation of membranous components of tissue. Hemosiderin deposition in human brain often accompanies chronic posttraumatic seizures induced by trauma. We injected an aqueous solution of iron salts, the principal metallic ion of whole blood, into rat isocortex. Serial electroencephalographic recording showed that 94% of untreated animals developed epileptiform discharges. Pretreatment with a-tocopherol and with 2 ppm selenium prevented development of iron-induced epileptiform activity in 72% of animals. Histopathologic assessment of serial sections stained with Nissl, hematoxylin and eosin, and prussian blue showed cavitation, neuronal pyknosis and loss, and astrogliosis in untreated animals. The site of iron injection in animals treated with antiperoxidants contained only an area of neuronal pyknosis. The efficacy of antioxidants in preventing development of iron-induced cavitation, gliosis, and epileptiform discharges suggests that peroxidative injury may be important in the development of experimental epilepsy induced by isocortical injection of ferrous chloride.