Effect of host plant resistance to Tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector
Effect of host plant resistance to Tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector
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DOI：
10.1094/phyto.2001.91.12.1209
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发表时间：
2001-12-01
期刊：
影响因子：
3.2
通讯作者：
Cohen, S
中科院分区：
文献类型：
--
作者：
Lapidot, M;Friedmann, M;Cohen, S
研究了番茄黄化曲叶病毒（TYLCV）感染抗病番茄植株对病毒流行的影响。四个番茄基因型表现出不同程度的病毒抗性，范围从完全易感到高抗性，作为TYLCV感染的源植物。评价了粉虱在不同来源植物上取食后的病毒获得率和传播率。TYLCV的传播率由粉虱感染源植物接种后21天（DPI），不久后出现TYLCV症状，呈负相关，与源植物显示的抗性水平。因此，电阻越高，传输速率越低。此外，与感病植物相比，TYLCV DNA积累在抗性源植物中显示较低。粉虱存活率，源植物21 DPI喂养后，是相似的所有品种测试。然而，在35 DPI时取食受感染的源植物后，发现粉虱存活率存在显著差异;在此，粉虱存活率随着源植物显示出的抗性水平的提高而增加。在35 DPI时，易感植物已经发展出严重的TYLCV疾病症状，并且来自这些植物的传播率是最低的，推测是由于这些植物的不良状况。传播率从源植物显示中等水平的电阻水平是最高的，与利率下降源植物显示较高水平的TYLCV电阻喂养。在21和35 DPI时取食感染的源植物后，粉虱中TYLCV DNA的积累与源植物中病毒DNA的积累直接相关。结果表明，从本质上讲，抗性表达越高，植物越不适合作为病毒稀缺品。因此，在从高抗性植物获得后，粉虱传播TYLCV的效率将较低。
The effect that Tomato yellow leaf curl virus (TYLCV)-infected resistant tomato plants may have on virus epidemiology was studied. Four tomato genotypes that exhibit different levels of viral resistance, ranging from fully susceptible to highly resistant, served as TYLCV-infected source plants. Viral acquisition and transmission rates by whiteflies following feeding on the different source plants were evaluated. TYLCV transmission rate by whiteflies that had fed on infected source plants 21 days postinoculation (DPI), shortly after the appearance of TYLCV symptoms, was negatively correlated with the level of resistance displayed by the source plant. Therefore, the higher the resistance, the lower the transmission rate. In addition, TYLCV DNA accumulation was shown to be lower in the resistant source plants compared with the susceptible plants. Whitefly survival rate, following feeding on source plants 21 DPI, was similar for all the cultivars tested. Significant differences in whitefly survival were found, however, following feeding on the infected source plants at 35 DPI; here, whitefly survival rate increased with higher levels of resistance displayed by the source plant. At 35 DPI, the susceptible plants had developed severe TYLCV disease symptoms, and transmission rates from these plants were the lowest, presumably due to the poor condition of these plants. Transmission rates from source plants displaying a medium level of resistance level were highest, with rates declining following feeding on source plants displaying higher levels of TYLCV resistance. TYLCV DNA accumulation in whiteflies following feeding on infected source plants at both 21 and 35 DPI was directly correlated with viral DNA accumulation in source plants. Results show that, in essence, the higher the resistance expressed, the less suitable the plant was as a viral scarce. Consequently, following acquisition from a highly resistant plant, TYLCV transmission by whiteflies will be less efficient.