Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): Proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. nov as Lelliottia nimipressuralis comb. nov and Lelliottia amnigena comb. nov., respectively, E. gergoviae and E. pyrinus into Pluralibacter gen. nov as Pluralibacter gergoviae comb. nov and Pluralibacter pyrinus comb. nov., respectively, E. cowanii, E. radicincitans, E. oryzae and E. arachidis into Kosakonia gen. nov as Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov and Kosakonia arachidis comb. nov., respectively, and E. turicensis, E. helveticus and E. pulveris into Cronobacter as Cronobacter zurichensis nom. nov., Cronobacter helveticus comb. nov and Cronobacter pulveris comb. nov., respectively, and emended description of the genera Enterobacter and Cronobacter
Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): Proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. nov as Lelliottia nimipressuralis comb. nov and Lelliottia amnigena comb. nov., respectively, E. gergoviae and E. pyrinus into Pluralibacter gen. nov as Pluralibacter gergoviae comb. nov and Pluralibacter pyrinus comb. nov., respectively, E. cowanii, E. radicincitans, E. oryzae and E. arachidis into Kosakonia gen. nov as Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov and Kosakonia arachidis comb. nov., respectively, and E. turicensis, E. helveticus and E. pulveris into Cronobacter as Cronobacter zurichensis nom. nov., Cronobacter helveticus comb. nov and Cronobacter pulveris comb. nov., respectively, and emended description of the genera Enterobacter and Cronobacter
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DOI：
10.1016/j.syapm.2013.03.005
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发表时间：
2013-07-01
影响因子：
3.4
通讯作者：
De Vos, Paul
中科院分区：
文献类型：
--
作者：
Brady, Carrie;Cleenwerck, Ilse;De Vos, Paul
肠杆菌的分类有一个复杂的历史，有几个种在这个属之间转移。根据16S rRNA基因序列，菌株的分类是困难的，因为它是多种多样的。以前人们已经承认，肠杆菌中含有应该转移到其他属的物种。为了解决肠杆菌的分类问题，基于蛋白编码基因(gyrB、rpoB、infB和atpD)的部分序列，对肠杆菌、克隆氏菌和沙雷氏菌的模式菌株和参考菌株以及与之密切相关的柠檬酸杆菌属、克雷伯氏杆菌属、克鲁维拉杆菌属、莱克雷氏菌属、芒格氏杆菌属、拉氏杆菌属和约肯氏杆菌属的成员进行了MLSA分析。对串联核苷酸序列的系统发育分析表明，肠杆菌可分为5个强烈支持的MLSA类群，这表明该物种应重新划分为5个不同的属。连接的氨基酸树、表型特征和脂肪酸图谱为这一点提供了进一步的支持，使MLSA组得以区分。提出了3个新属：Lelliottia gen.Nov.，Pluralibacter gen.11月和Kosakonia gen.11月和以下新组合：尼玛水母Lelliottia nimipressuris comb.新种Lelliottia amnigena comb.新种Pluralibacter gergoviae comb.，Pluralibacter pluralibacter pyrinus comb.Nov.、科萨科尼亚Covanii Comb.Nov.，Kosakonia Radincitans comb.Nov.，Kosakonia oryzae comb.Nov.，Kosakonia arachidis comb.、Helveticus Cronbacter helveticus comb.11月和粉状冠状芽孢杆菌(Cronbacter grveris comb.)11月此外，还发现了一种新的称谓--苏云金芽孢杆菌。11月建议将屠宰肠杆菌重新分类为慢杆菌属，因为屠宰慢杆菌已经在使用中(Iversen等人，2008年)。(C)2013年爱思唯尔股份有限公司。版权所有。
The taxonomy of Enterobacter has a complicated history, with several species transferred to and from this genus. Classification of strains is difficult owing to its polyphyletic nature, based on 16S rRNA gene sequences. It has been previously acknowledged that Enterobacter contains species which should be transferred to other genera. In an attempt to resolve the taxonomy of Enterobacter, MLSA based on partial sequencing of protein-encoding genes (gyrB, rpoB, infB and atpD) was performed on the type strains and reference strains of Enterobacter, Cronobacter and Serratia species, as well as members of the closely related genera Citrobacter, Klebsiella, Kluyvera, Leclercia, Mangrovibacter, Raoultella and Yokenella. Phylogenetic analyses of the concatenated nucleotide sequences revealed that Enterobacter can be divided into five strongly supported MLSA groups, suggesting that the species should be reclassified into five different genera. Further support for this was provided by a concatenated amino acid tree, phenotypic characteristics and fatty acid profiles, enabling differentiation of the MLSA groups. Three novel genera are proposed: Lelliottia gen. nov., Pluralibacter gen. nov. and Kosakonia gen. nov. and the following new combinations: Lelliottia nimipressuralis comb. nov., Lelliottia amnigena comb. nov., Pluralibacter gergoviae comb. nov., Pluralibacter pyrinus comb. nov., Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov., Kosakonia arachidis comb. nov., Cronobacter helveticus comb. nov. and Cronobacter pulveris comb. nov. Additionally, the novel epithet Cronobacter zurichensis nom. nov. is proposed for the reclassification of Enterobacter turicensis into the genus Cronobacter, as Cronobacter turicensis (Iversen et al., 2008) is already in use. (C) 2013 Elsevier GmbH. All rights reserved.