Enhanced recovery of phenolic compounds from bitter melon (Momordica charantia) by subcritical water extraction
Enhanced recovery of phenolic compounds from bitter melon (Momordica charantia) by subcritical water extraction
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DOI：
10.1016/j.seppur.2008.11.014
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发表时间：
2009-04-07
影响因子：
8.6
通讯作者：
Shotipruk, Artiwan
中科院分区：
文献类型：
--
作者：
Budrat, Parichat;Shotipruk, Artiwan
采用亚临界水萃取法（SWE）提高了苦瓜（Momordica charantia）中酚类化合物的回收率。在恒压10 MPa条件下进行提取，考察了提取温度（130 ~ 200℃）和水流速（2 ~ 5 ml/min）对酚类化合物提取率的影响，确定了提取温度150 ~ 200℃、流速2 ml/min为最佳提取条件。SWE提取液中酚类化合物的含量（52.63 mg没食子酸当量(GAE)/g干重）显著高于甲醇提取液（5.00 ~ 6.00 mg GAE/g DW）和索氏水提取液（5.00 ~ 7.00 mg GAE/g DW）。苦瓜中酚酸含量最高的是儿茶素，SWE提取、MeOH提取和索氏提取的儿茶素含量分别为46.16、1.61和1.77 mg/g DW。低温SWE提取液中酚类化合物的含量低于高温SWE提取液，但具有较高的抗氧化活性。然而，SWE提取的所有提取物的抗氧化活性都比在常压条件下用甲醇或水提取的提取物高约三倍。(C) 2008 Elsevier B.V.版权所有
In this study, enhanced recovery of phenolic compounds from bitter melon (Momordica charantia) was achieved by subcritical water extraction (SWE). The extraction was carried out at a constant pressure of 10 MPa and the effects of extraction temperature (130-200 degrees C) and water flow rate (2-5 ml/min) were determined on the amount of phenolic compounds and the most suitable extraction condition was found to be at the temperature between 150 and 200 degrees C and at the flow rate of 2 ml/min. The amount of phenolic compounds in the extracts obtained by SWE (52.63 mg gallic acid equivalents (GAE)/g dry weight (DW)) was significantly higher than those in the extracts obtained by MeOH extraction (5.00-6.00 mg GAE/g DW) and soxhlet water extraction (5.00-7.00 mg GAE/g DW). The most phenolic acids contained in bitter melon were catechin, whose content in the extracts obtained by the SWE, MeOH extraction, and soxhlet extraction were 46.16, 1.61, and 1.77 mg/g DW, respectively. Although the amount of phenolic compounds in the extracts obtained by SWE at lower temperature was found to be lower than those obtained at higher temperature, they exhibited higher antioxidant activity. Nevertheless, all extracts obtained with SWE had approximately threefold higher antioxidant activity than those obtained by extraction with MeOH or water at ambient pressure conditions. (C) 2008 Elsevier B.V. All rights reserved.