Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues
Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues
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DOI：
10.1159/000094801
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Yasue, H.
中科院分区：
文献类型：
--
作者：
Kiuchi, S.;Yamada, T.;Yasue, H.
Taste receptor family 1 member 3，TAS 1 R3，在小鼠中参与甜味和鲜味，该基因的核苷酸序列已在大鼠、大猩猩和人类中报道。猪经常被用作人类疾病的模型，并且由于其与人类的解剖学和生理学相似性，也被认为是异种移植到人类的源动物。因此，在本研究中，确定了猪TAS 1 R3基因的基因组结构，并研究了TAS 1 R3在各种猪组织中的表达。该基因位于猪染色体6 q22-> q23上，从该染色体上产生了3种类型的mRNA：来自舌的6个外显子的3，752 bp、来自6个外显子的3，704 bp和来自睾丸的7个外显子的3，630 bp。TAS 1 R3的6个外显子/5个内含子结构与人和小鼠的相似，但7个外显子/6个内含子结构首次在猪中观察到。实时荧光定量PCR结果显示TAS 1 R3在舌、肾和睾丸中有高表达。除肾脏外，其他组织的表达谱与小鼠相似。在猪舌和睾丸组织上进行使用TAS 1 R3的RNA探针的原位杂交时，发现TAS 1 R3在舌轮廓乳头、菌状乳头、粘膜上皮、滤泡B淋巴细胞、舌扁桃体粘膜下组织中的淋巴细胞和生精细胞中表达。使用外周成熟B淋巴细胞，通过实时PCR和实时PCR产物的测序进一步证实TAS 1 R3在B淋巴细胞中的表达。
Taste receptor family 1 member 3, TAS1R3, is shown to be involved in sweet and umami tastes in mouse, and the nucleotide sequence of the gene has been reported in rat, gorilla, and human. Pigs are frequently used as models for human diseases, and are also considered to be source animals for xenotransplantation to humans due to their anatomical and physiological similarities to humans. Therefore, in the present study, the genomic structure of the swine TAS1R3 gene was determined, and TAS1R3 expression was studied in various swine tissues. The gene was shown to reside on swine chromosome 6q22 -> q23, from which three types of mRNAs were generated: 3,752 bp derived from six exons in tongue, 3,704 bp from six exons and 3,630 bp from seven exons in testis. The 6 exons/5 introns were structurally similar to those of humans and mice, but the 7 exons/6 introns structure of TAS1R3 was first observed in swine. High expressions of TAS1R3 were revealed in tongue, kidney, and testis by real-time PCR. The expression profile of the tissues except for kidney was similar to that of mouse. When in situ hybridization using an RNA probe for TAS1R3 was performed on swine tongue and testis tissues, TAS1R3 expressions were revealed in tongue circumvallate papillae, fungiform papillae, mucosal epithelium, follicular B lymphocytes, lymphocytes in submucosal tissues of lingual tonsil, and spermatogenic cells. Using peripheral mature B lymphocytes, the expression of TAS1R3 in B lymphocytes was further confirmed by real-time PCR and sequencing of the real-time PCR product.