The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage
The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage
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DOI：
10.1016/j.biomaterials.2010.02.028
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发表时间：
2010-06-01
期刊：
影响因子：
14
通讯作者：
Hoshi, Kazuto
中科院分区：
文献类型：
--
作者：
Tanaka, Yoko;Yamaoka, Hisayo;Hoshi, Kazuto
为了扩大软骨再生医学的临床应用，需要开发一种具有坚固和三维结构的植入型组织工程软骨。为此，我们尝试将多孔可生物降解聚合物支架与胶原水凝胶相结合，并对多孔支架的结构和组成进行了优化。我们将软骨细胞/胶原混合物植入不同孔隙率(80-95%)和孔径(0.3-2.0 mm)的PLLA或相关聚合物(PDLA、PLLA/CL和PLGA)的支架中，并将构建的支架移植到裸鼠皮下。使用超大孔径(>；1 mm)支架的构建物在皮肤上破裂，损害宿主组织。孔隙率为95%、孔径为0.3 mm的支架能有效地保留细胞/凝胶混合物，显示软骨再生良好。在组成方面，PLGA和PLLA的组织工程软骨优于PLGA/CA和PDLA。后两者表现为巨噬细胞密集堆积，可能影响软骨再生。尽管目前推荐将PLGA或PLLA作为软骨支架材料，但生物降解与软骨再生完全同步的聚合物将提高组织工程软骨的质量。(C)2010爱思唯尔有限公司。保留所有权利。
To broaden the clinical application of cartilage regenerative medicine, we should develop an implant-type tissue-engineered cartilage with firmness and 3-D structure. For that, we attempted to use a porous biodegradable polymer scaffold in the combination with atelocollagen hydrogel, and optimized the structure and composition of porous scaffold. We administered chondrocytes/atelocollagen mixture into the scaffolds with various kinds of porosities (80-95%) and pore sizes (0.3-2.0 mm), consisting of PLLA or related polymers (PDLA, PLA/CL and PLGA), and transplanted the constructs in the subcutaneous areas of nude mice. The constructs using scaffolds of excessively large pore sizes (>1 mm) broke out on the skin and impaired the host tissue. The scaffold with the porosity of 95% and pore size of 0.3 mm could effectively retain the cells/gel mixture and indicated a fair cartilage regeneration. Regarding the composition, the tissue-engineered cartilage was superior in PLGA and PLLA to that in PLA/CA and PDLA. The latter two showed the dense accumulation of macrophages, which may deteriorate the cartilage regeneration. Although PLGA or PLLA has been currently recommended for the scaffold of cartilage, the polymer for which biodegradation was exactly synchronized to the cartilage regeneration would improve the quality of the tissue-engineered cartilage. (C) 2010 Elsevier Ltd. All rights reserved.