Whi7/Srl3 polymorphisms reveal its role in cell size and quiescence.
Whi7/Srl3 polymorphisms reveal its role in cell size and quiescence.
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DOI：
10.17912/micropub.biology.000661
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发表时间：
2022
影响因子：
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通讯作者：
Breeden, Linda L
中科院分区：
文献类型：
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作者：
Miles, Shawna;Breeden, Linda L
Whi 5和Sr 13/Whi 7是由S.酿酒酵母（Wolfe和Shields 1997）。Whi 5在结合和抑制晚期G1转录中发挥Rb样功能，该转录促进从G1到S的进展（Costanzo et al. 2004; de Bruin et al. 2004）。Whi 7也可以与G1转录复合物结合，并在过度产生时促进G1阻滞（Gomar-Alba et al. 2017），但其转录主要由应激诱导（Ragni et al. 2011; Mendez et al. 2020）。我们已经使用多态性在两个实验室酵母菌株，发现新的功能Whi 7在日志和静止细胞。这些包括小细胞大小在日志阶段和缺陷的进入，维护和恢复从静止。
Whi5 and Srl3/Whi7 are related proteins that resulted from the whole genome duplication of S. cerevisiae (Wolfe and Shields 1997). Whi5 plays an Rb-like function in binding and inhibiting the late G1 transcription that promotes progression from G1 to S (Costanzo et al. 2004; de Bruin et al. 2004). Whi7 can also associate with G1 transcription complexes and promotes G1 arrest when overproduced (Gomar-Alba et al. 2017), but its transcription is primarily induced by stress (Ragni et al. 2011; Mendez et al. 2020). We have used polymorphisms in two laboratory yeast strains to uncover novel functions of Whi7 in log and quiescent cells. These include small cell size during log phase and defects in entry, maintenance and recovery from quiescence.