Freeze-induced shrinkage of individual cells and cell-to-cell propagation of intracellular ice in cell chains from salivary glands
Freeze-induced shrinkage of individual cells and cell-to-cell propagation of intracellular ice in cell chains from salivary glands
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DOI：
10.1007/bf01938868
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Uhrik, B
中科院分区：
文献类型：
--
作者：
Berger, WK;Uhrik, B
细胞内冰（IIF）的形成，通常是一个致命的事件，他避免冷冻保存细胞时，应，然而，在冷冻手术破坏肿瘤细胞。迄今为止，IIF仅在悬浮液中的单细胞中进行了研究。由于组织中的细胞不能成功地冷冻保存，与悬浮液中的单细胞相反，组织中IIF的机制可能取决于促进IIF的因素。我们研究了来自唾液腺的细胞链中的IIF，唾液腺代表了一种简单的组织形式。它们的细胞通过负责细胞间通讯的通道连接。在冷冻过程中，相当一部分细胞脱水发生在细胞被冰包裹之前，这种冰前收缩的程度似乎会影响IIF。在链与耦合的细胞IIF传播从一个细胞到相邻的细胞在一个连续的方式与短的延迟（200-300毫秒），这表明细胞间传播通过细胞间通道。在用解耦剂（二硝基苯酚，庚醇）预处理的链中，不存在顺序IIF。相反，冰的形成是随机的，在连续的变暗之间有更长和可变的延迟，表明IIF。结果表明，IIF传播的机制，并因此在组织中的冷冻损伤的程度，可以通过细胞间通道（缝隙连接）的存在下的影响。
The formation of intracellular ice (IIF), usually a lethal event to he avoided when cryopreserving cells, should, however, be enforced during the cryosurgical destruction of tumour cells. IIF has been investigated so far only in single cells in suspension. Because cells in tissues cannot be successfully cryopreserved, in contrast to single cells in suspension, the mechanism of IIF in tissues may depend on factors that facilitate IIF. We studied IIF in cell strands from salivary glands, which represent a simple form of a tissue. Their cells are connected by channels responsible for intercellular communication. A substantial fraction of cell dehydration during freezing occurs before cells are encapsulated by ice, and the degree of this pre-ice-front shrinkage appears to influence IIF. In strands with coupled cells IIF spread from one cell to adjacent cells in a sequential manner with short delays (200-300 ms), suggesting cell-to-cell propagation via intercellular channels. In strands pretreated with decoupling agents (dinitrophenol, heptanol), sequential IIF was absent. Instead, formation of ice was random, with longer and variable delays between consecutive darkenings indicating IIF. Results suggest that the mechanism of IIF spread, and consequently the degree of cryodamage in tissue, can be influenced by the presence of intercellular channels (gap junctions).