ISOLATION AND CHARACTERIZATION OF CALCIFYING MATRIX VESICLES FROM EPIPHYSEAL CARTILAGE
ISOLATION AND CHARACTERIZATION OF CALCIFYING MATRIX VESICLES FROM EPIPHYSEAL CARTILAGE
复制标题
DOI：
10.1073/pnas.67.3.1513
复制
发表时间：
1970-01-01
影响因子：
11.1
通讯作者：
ANDERSON, HC
中科院分区：
文献类型：
--
作者：
ALI, SY;SAJDERA, SW;ANDERSON, HC
基质囊泡与软骨中的初始钙化有关，已从牛胚胎骨骺软骨中分离出来。用胶原酶消化软骨，差速离心法将软骨分成7个组分。细胞部分含有消化中80%以上的DNA。含有基质囊泡的胞外部分对碱性磷酸酶、焦磷酸酶、ATPase和5‘-AMPase的比活力也最高(EC分别为3.1.3.1、3.6.1.1、3.6.1.3和3.1.3.5)。另一方面，酸性磷酸酶(EC 3.1.3.2)的活性主要存在于细胞组分中，这表明基质囊泡与溶酶体是截然不同的。这似乎是第一次从正常组织中分离出膜结合的细胞外颗粒。基质囊泡含有酶，可以增加正磷酸盐的局部浓度，从而可能导致羟基磷灰石的形成。膜结合的基质小泡还可以提供一种机制，使依赖于ATP的钙或磷运输到小泡的管腔内，从而导致矿化。
Matrix vesicles, associated with initial calcification in cartilage, have been isolated from bovine fetal epiphyseal cartilage. Cartilage was digested with collagenase, then partitioned into seven fractions by differential centrifugation. The cellular fractions contained over 80% of the DNA in the digest. The extracellular fraction that contained matrix vesicles, in which apatite crystals were often seen on electron microscopy, also displayed the highest specific activity for alkaline phosphatase, pyrophosphatase, ATPase, and 5′-AMPase (EC 3.1.3.1., 3.6.1.1, 3.6.1.3, and 3.1.3.5, respectively). Most of the acid phosphatase (EC 3.1.3.2) activity, on the other hand, was found in the cellular fractions, indicating that matrix vesicles are quite distinct from lysosomes. This appears to be the first instance of isolation of membrane-bounded extracellular particles from any normal tissue.The matrix vesicles possess enzymes that can increase the local concentration of orthophosphate and thus could lead to the formation of hydroxyapatite. The membrane-bounded matrix vesicles may also provide a mechanism for ATP-dependent transport of calcium or phosphate into the lumen of the vesicles with resultant mineralization.