INHIBITION OF POLY(ADP-RIBOSE) SYNTHETASE BY UNSATURATED FATTY-ACIDS, VITAMINS AND VITAMIN-LIKE SUBSTANCES
INHIBITION OF POLY(ADP-RIBOSE) SYNTHETASE BY UNSATURATED FATTY-ACIDS, VITAMINS AND VITAMIN-LIKE SUBSTANCES
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DOI：
10.1016/0014-5793(90)81378-2
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发表时间：
1990-04-24
期刊：
影响因子：
3.5
通讯作者：
UEDA, K
中科院分区：
文献类型：
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作者：
BANASIK, M;KOMURA, H;UEDA, K
多种维生素及维生素样物质对体外聚腺苷磷酸核糖合成酶活性有抑制作用。最有效的是必需脂肪酸，即花生四烯酸、亚油酸和亚麻酸；其50%抑制浓度（IC50）为44 ~ 110 μM，效价高于维生素抑制剂烟酰胺（IC50= 210 μM）。其次是维生素K3、K1和视网膜，其次是α-硫辛酸、辅酶Q10和5-磷酸吡哆醛。烟酰胺和维生素K3对NAD+的抑制表现为混合型抑制，花生四烯酸对NAD+的抑制表现为50 μM的竞争性抑制和100 μM的混合型抑制。
Various vitamins and vitamin-like substances inhibited the activity of poly(ADP-ribose) synthetase in vitro. The most potent were essential fatty acids, i.e. arachidonic acid, linoleic acid, and linolenic acid; their 50% inhibitory concentrations (IC50) were 44–110 μM, indicating a higher potency than nicotinamide, a well-known vitamin inhibitor (IC50= 210 μM). Vitamins K3, K1, and retinal were the next strongest inhibitors, followed by α-lipoic acid, coenzyme Q10, and pyridoxal 5-phosphate. Nicotinamide and vitamin K3, exhibited mixed-type inhibition with respect to NAD+,while arachidonic acid exhibited dual inhibitions, competitive at 50 μM and mixed-type at 100 μM.