Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy
Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy
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DOI：
10.1364/josaa.19.001910
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发表时间：
2002-09-01
影响因子：
1.9
通讯作者：
Stelzer, EHK
中科院分区：
文献类型：
--
作者：
Swoger, J;Martínez-Corral, M;Stelzer, EHK
光学扫描全息术（奥什）的生物应用的显微成像领域的适用性进行了评估。一个广义的数学描述的奥什，考虑到偏振效应，高数值孔径，和广义照明波前。该描述用于表明，与传统的双光束扫描方法相比，所提出的单光束扫描技术放宽了奥什正确运行的限制。它还表明，虽然在一般奥什仅限于薄的样品，这种情况下可以放宽在nonrefracting荧光样品，这是在生物显微镜的重要性。(C)2002年美国光学学会。
The applicability of optical scanning holography (OSH) to the field of microscopic imaging for biological applications is assessed. A generalized mathematical description of OSH that takes into account polarization effects, high numerical apertures, and generalized illumination wave fronts is presented. This description is used to show that the proposed single-beam scanning technique relaxes the restrictions under which OSH functions correctly compared with the conventional double-beam scanning method. It is also shown that, although in general OSH is restricted to thin samples, this condition can be relaxed in nonrefracting fluorescence samples, which are of importance in biological microscopy. (C) 2002 Optical Society of America.