Single and joint toxic effects of four antibiotics on some metabolic pathways of zebrafish (Danio rerio) larvae
Single and joint toxic effects of four antibiotics on some metabolic pathways of zebrafish (Danio rerio) larvae
复制标题
四种抗生素对斑马鱼幼虫某些代谢途径的单一和联合毒性作用
DOI：
10.1016/j.scitotenv.2020.137062
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发表时间：
2020
影响因子：
9.8
通讯作者：
Zheng Chunmiao
中科院分区：
文献类型：
--
作者：
Qiu Wenhui;Liu Xinjie;Yang Feng;Li Rongzhen;Xiong Ying;Fu Caixia;Li Guanrong;Liu Shuai;Zheng Chunmiao
在中国，抗生素常用于人类和兽药，并存在于各种环境介质中。因此，抗生素对生物体的毒性作用引起了社会和科学家的关注。本研究利用斑马鱼胚胎，结合实验和组学技术的结果，测试了磺胺间甲氧嘧啶（SMM）、头孢噻肟钠（CFT）、四环素（TC）、恩诺沙星（ENR）及其组合四种抗生素的单一和联合毒性。接触抗生素120 h后，所有100 μg/L抗生素组的斑马鱼幼虫体长均显着缩短，100 μg/L Mix组的活性氧（ROS）含量显着增加。转录组测序 (RNA-seq) 显示，五个抗生素治疗组中许多基因的 mRNA 水平发生了显着变化。京都基因和基因组百科全书（KEGG）对差异表达基因的富集分析揭示了类固醇生物合成和其他代谢途径的显着富集。 Hub基因分析突出了dhcr24、acat1、aldh1a2、aldh8a1、suclg2、hadh和hsdl2为关键基因，抗生素处理导致的hub基因表达变化表明斑马鱼幼虫的代谢系统因与其他基因的相互作用而受到严重破坏。总之，在环境浓度下单独或联合暴露于不同抗生素会影响斑马鱼幼虫的早期发育和代谢系统，我们的结果为未来水生生物中抗生素毒性的研究提供了基础证据。
In China, antibiotics are commonly used for human and veterinary medicine, and they are present in various environmental media. Thus, the toxic effects of antibiotics on organisms have attracted the attention of society and scientists alike. In this study, zebrafish embryos were used to test the single and joint toxicity of four antibiotics, sulfamonomethoxine (SMM), cefotaxime sodium (CFT), tetracycline (TC), enrofloxacin (ENR), and their combinations, combining the results of experimental and omics techniques. Following exposure to antibiotics for 120 h, the body lengths of zebrafish larvae in all 100 μg/L antibiotic groups were significantly shortened, and the reactive oxygen species (ROS) content in the 100 μg/L Mix group was significantly increased. Transcriptome sequencing (RNA-seq) showed that the mRNA level of numerous genes was significantly changed in the five antibiotic treatment groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes revealed a significant enrichment of the steroid biosynthesis and other metabolism pathways. Hub gene analysis highlighteddhcr24,acat1,aldh1a2,aldh8a1,suclg2,hadh, andhsdl2as the key genes, and hub gene expression changes because of the antibiotic treatment suggested that the metabolic system of the zebrafish larvae was severely disrupted by the interaction with other genes. In conclusion, single or joint exposure to different antibiotics at environmental concentrations affected the early development and metabolic system of zebrafish larvae, and our results provide fundamental evidence for future studies of antibiotic toxicity in aquatic organisms.