'Genotypic shuffling' of sequential clones of Candida albicans in HIV-infected individuals with and without symptomatic oral candidiasis
'Genotypic shuffling' of sequential clones of Candida albicans in HIV-infected individuals with and without symptomatic oral candidiasis
复制标题
DOI：
10.1099/jmm.0.04972-0
复制
发表时间：
2003-04-01
影响因子：
3
通讯作者：
Yeung, KWS
中科院分区：
文献类型：
--
作者：
Samaranayake, YH;Samaranayake, LP;Yeung, KWS
尽管hiv感染的个体携带多种口腔白色念珠菌菌株，但人们对它们随时间的微进化知之甚少。因此，我们对16名有或无口咽念珠菌病症状的HIV感染者进行了一项前瞻性研究，以评估HIV疾病进展期间口腔白色念珠菌分离株的基因型分布。在12个月的多次访问期间，从所有个体中获取口腔冲洗液样本，每次访问最多选择5个白色念珠菌菌落。采用标准真菌学标准鉴定分离株后，采用随机扩增多态性DNA （RAPID）技术对白念珠菌序列克隆内和序列克隆间酵母菌分离株的遗传相似性进行鉴定。利用市售软件对每个分离株的快速凝胶图谱和谱系进行进一步分析。快速研究显示，在研究期间，每个个体的流行率高达14种不同的基因型，从一次口腔冲洗液中同时分离出多种基因型。对RAPID图谱的计算机分析显示，与从无症状个体分离的酵母菌相比，从有症状个体连续访问中分离的酵母菌表现出惊人的亲近性。遗传上相同的白色念珠菌菌株也形成了“松散”连接的亚簇，在多次访问中重叠，这意味着这些分离株在疾病进展过程中存在遗传“洗牌”。这些数据表明，与有症状的口腔念珠菌病和HIV无症状携带者相关的白色念珠菌有不同的进化遗传趋势。
Although HIV-infected individuals harbour multiple strains of oral Candida albicans, little is known of their micro-evolution over time. Therefore, a prospective study was conducted with 16 HIV-infected ethnic Chinese individuals with and without symptoms of oropharyngeal candidiasis to evaluate the genotype distribution of oral C. albicans isolates during HIV disease progression. Oral-rinse samples were obtained from all individuals and up to five C. albicans colonies were selected for each visit, over a 12 month period of multiple visits. After identification of isolates using standard mycological criteria, the genetic similarities of yeast isolates within and between sequential clones of C. albicans were assessed by DNA fingerprinting through random amplification of polymorphic DNA (RAPID). The results of RAPID gel profiles and the lineage of each isolate were further analysed using commercially available software. RAPID studies revealed the prevalence of up to 14 different genotypes per individual during the study period, with multiple genotypes isolated simultaneously from a single oral rinse. Computer analysis of RAPID profiles revealed that yeasts isolated over sequential visits from symptomatic individuals demonstrated a striking level of relatedness compared with isolates from asymptomatic individuals. Genetically identical C. albicans strains also formed 'loosely' connected subclusters that overlapped multiple visits, implying genetic 'shuffling' in these isolates during disease progression. These data point to varying evolutionary genetic trends in C. albicans associated with symptomatic oral candidiasis and asymptomatic carriage in HIV disease.