Subset of cells immunopositive for neurokinin-1 receptor identified as arterial interstitial cells of Cajal in human large arteries
Subset of cells immunopositive for neurokinin-1 receptor identified as arterial interstitial cells of Cajal in human large arteries
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DOI：
10.1007/s00441-004-1061-9
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Bobryshev, YV
中科院分区：
文献类型：
--
作者：
Bobryshev, YV
在大动脉外膜，树突状细胞位于神经纤维之间，其中一些含有p物质。本研究的目的是检测神经激肽1受体（NK-1R）是否由动脉壁的树突状细胞表达。用抗nk - 1r和细胞类型特异性抗体对主动脉和颈动脉段平行切片进行免疫染色。动脉壁树突状细胞表达NK-1R，但表达水平较低。其他强烈表达NK-1R的细胞位于中膜和外膜之间的边界。它们不共表达任何树突状细胞标记物，包括束蛋白、CD1a、S100或lag抗原，并且CD68、CD3和肥大细胞胰蛋白酶呈阴性。对这些NK-1R（+）停止细胞进行激光捕获显微解剖和电镜分析。微解剖细胞与神经末梢直接接触，超微结构具有胃肠道Cajal间质细胞的典型特征。进一步的系统电镜分析显示，具有Cajal间质细胞特征的细胞是人类动脉壁结构的基本元素。Cajal动脉间质细胞c-kit阴性，但表达血管活性肠肽受体1 （VIPR1）。在动脉粥样硬化病变的动脉段中，观察到Cajal动脉间质细胞与神经末梢之间接触的破坏性改变。Cajal动脉间质细胞的功能意义及其在动脉粥样硬化和其他血管疾病中的可能参与有待澄清。
In the adventitia of large arteries, dendritic cells are located between nerve fibers, some of which contain substance P. The aim of the present study was to examine whether neurokinin 1 receptor (NK-1R) was expressed by dendritic cells in the arterial wall. Parallel sections of aortic and carotid artery segments were immunostained with anti-NK-1R and cell-type-specific antibodies. Dendritic cells in the arterial wall expressed NK-1R, albeit at a low level. Other cells, which intensely expressed NK-1R, were located along the border between the media and adventitia. They did not co-express any dendritic cell markers, including fascin, CD1a, S100, or Lag-antigen, and were negative for CD68, CD3, and mast cell tryptase. These NK-1R(+) stop cells were laser-capture microdissected and studied by means of electron-microscopic analysis. The microdissected cells were in direct contact with nerve endings, and their ultrastructure was typical of the interstitial cells of Cajal present in the gastrointestinal tract. Further systematic electron-microscopic analysis revealed that the cells displaying the features typical of interstitial cells of Cajal were a basic element of the human arterial wall architectonics. Arterial interstitial cells of Cajal were negative for c-kit but they expressed vasoactive intestinal peptide receptor 1 (VIPR1). Destructive alterations of contacts between arterial interstitial cells of Cajal and nerve endings were observed in arterial segments with atherosclerotic lesions. The functional significance of the arterial interstitial cells of Cajal and their possible involvement in atherosclerosis and other vascular diseases need clarification.