Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches
Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches
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DOI：
10.1073/pnas.071623998
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发表时间：
2001-03-27
影响因子：
11.1
通讯作者：
Georgiadis, RM
中科院分区：
文献类型：
--
作者：
Heaton, RJ;Peterson, AW;Georgiadis, RM
我们表明，在原位光学表面等离子体共振光谱可用于监测杂交动力学的未标记的DNA在拴系的单层核酸膜上的金在施加的静电场的存在下。电场可以增强或延迟杂交，也可以使表面固定的DNA双链体变性。通过简单地调整电极电位就可以区分匹配和失配的混合电路。虽然在界面处的电场是非常大的，拴系的单链DNA硫醇探针保持结合，并可以重复用于随后的杂交反应，而不损失效率。只有电容充电电流绘制;氧化还原反应是通过保持在理想的可极化区域内的金电极电位来避免的。由于电位引起的表面等离子体共振曲线的形状的变化，我们占整个曲线，而不是简单的共振最小值的移位。
We demonstrate that in situ optical surface plasmon resonance spectroscopy can be used to monitor hybridization kinetics for unlabeled DNA in tethered monolayer nucleic acid films on gold in the presence of an applied electrostatic field. The de field can enhance or retard hybridization and can also denature surface-immobilized DNA duplexes. Discrimination between matched and mismatched hybrids is achieved by simple adjustment of the electrode potential. Although the electric field at the interface is extremely large, the tethered single-stranded DNA thiol probes remain bound and can be reused for subsequent hybridization reactions without loss of efficiency. Only capacitive charging currents are drawn; redox reactions are avoided by maintaining the gold electrode potential within the ideally polarizable region. Because of potential-induced changes in the shape of the surface plasmon resonance curve, we account for the full curve rather than simply the shift in the resonance minimum.