Generation, expansion, and differentiation of cardiovascular progenitor cells from human pluripotent stem cells.
Generation, expansion, and differentiation of cardiovascular progenitor cells from human pluripotent stem cells.
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DOI：
10.1007/7651_2014_119
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Yang, Huang-Tian
中科院分区：
文献类型：
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作者：
Cao, Nan;Liang, He;Yang, Huang-Tian
来源于人胚胎干细胞和人诱导多能干细胞的心血管祖细胞（CVPC）是研究早期胚胎心血管发育和基于干细胞的先天性和获得性心脏病治疗的宝贵潜在来源。为了充分实现其价值，有必要建立一个有效和稳定的分化系统，用于将这些多能干细胞（PSC）诱导为CVPC并在培养中稳健地扩增它们，然后将这些CVPC进一步分化为多种心血管细胞类型。在这里，我们描述了在无饲养层和无血清培养条件下，在化学成分确定的培养基中从hPSC有效衍生、扩增和分化CVPC的方案。
Cardiovascular progenitor cells (CVPCs) derived from human embryonic stem cells and human induced pluripotent stem cells represent an invaluable potential source for the study of early embryonic cardiovascular development and stem cell-based therapies for congenital and acquired heart diseases. To fully realize their values, it is essential to establish an efficient and stable differentiation system for the induction of these pluripotent stem cells (PSCs) into the CVPCs and robustly expand them in culture, and then further differentiate these CVPCs into multiple cardiovascular cell types. Here we describe the protocols for efficient derivation, expansion, and differentiation of CVPCs from hPSCs in a chemically defined medium under feeder- and serum-free culture conditions.