Superoxide anion production is increased in a model of genetic hypertension - Role of the endothelium
Superoxide anion production is increased in a model of genetic hypertension - Role of the endothelium
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DOI：
10.1161/01.hyp.33.6.1353
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发表时间：
1999-06-01
期刊：
影响因子：
8.3
通讯作者：
Hamilton, CA
中科院分区：
文献类型：
--
作者：
Kerr, S;Brosnan, MJ;Hamilton, CA
在自发性高血压卒中倾向大鼠（SHRSP）中观察到的一氧化氮（NO）可用性降低是由于过量的超氧化物（O-2（-））的假设进行了检查。在12至16周的雄性和雌性Wistar-Kyoto大鼠（WKY）和SHRSP中研究了通过光泽精化学发光测量的O-2（-）产生。此外，研究了编码内皮NO合酶（参与NO生成的酶）的基因的表达。雄性和雌性SHRSP的O-2（-）生成量均增加（4.11+/-0.24和3.84+/-0.28 nmol O-2（-））。min（-1）。mg（-1）），且雄性WKY显著高于雌性WKY（雄性1.22+/-0.08和0.8+/-0.08 nmolO-2（-）.min（-1））。mg（-1））（SHRSP vs WKY P
The hypothesis that the decreased nitric oxide (NO) availability observed in spontaneously hypertensive stroke-prone rats (SHRSP) is due to excess superoxide (O-2(-)) was examined. O-2(-) generation, measured by lucigenin chemiluminescence, was studied in 12- to 16-week male and female Wistar-Kyoto rats (WKY) and SHRSP. In addition, expression of the gene encoding endothelial NO synthase, the enzyme involved in NO generation, was investigated. O-2(-) generation was increased in male and female SHRSP (4.11+/-0.24 and 3.84+/-0.28 nmol O-2(-) . min(-1) . mg(-1) respectively) compared with their WKY counterparts and was significantly higher in male than female WKY (1.22+/-0.08 in males and 0.8+/-0.08 nmol O-2(-) .min(-1) . mg(-1) respectively) (SHRSP versus WKY P