Strength, deformability and toughness of uncrosslinked fibrin fibers from theoretical reconstruction of stress-strain curves.
Strength, deformability and toughness of uncrosslinked fibrin fibers from theoretical reconstruction of stress-strain curves.
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DOI：
10.1016/j.actbio.2021.09.050
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发表时间：
2021-12
影响因子：
9.7
通讯作者：
Barsegov V
中科院分区：
文献类型：
--
作者：
Maksudov F;Daraei A;Sesha A;Marx KA;Guthold M;Barsegov V
纤维蛋白纤维的机械性能的结构机制是难以捉摸的。我们结合了体外和计算机模拟的未交联纤维蛋白聚合物的拉伸试验，以探索其材料特性。纤维蛋白纤维的实验应力（σ）-应变（ε）曲线的特征在于弹性变形，当ε<160%时，由于αC系链的解开和纤维蛋白原纤维的拉直，弹性响应较弱，而当ε>160%时，由于纤维蛋白单体中的卷曲螺旋和γ结节的展开，弹性响应较强。应变ε>212%时纤维断裂是由于球孔键的解离和D：D界面的断裂。我们开发了波动双线性弹簧模型来解释σ - ε曲线，根据原纤维排列的自由能Δ G 0 = 10.1-11.5 kBT，原纤维排列的杨氏模量Yu = 1.9-3.2 MPa和拉伸Ya = 5.7-9.7 MPa，纤维断裂的应变尺度和原纤维协同性m = 3.6-8。我们应用该模型表征了纤维强度σcr = 12-13 MPa，变形能力εcr = 222%，断裂韧性U = 9 MJ/m3，并求解了热力学状态函数，其中原纤维排列（室温）的熵变为96.9 GJ/mol，原纤维拉伸的焓变为113.6 GJ/mol，其加起来的自由能变化为210.5 GJ/mol。纤维伸长与原纤维脱水和滑动机制相关，以产生有序的原纤维阵列。纤维的行为类似于水凝胶;原纤维脱水和水排出占纤维伸长和断裂的总自由能变化的约94-98%。
Structural mechanisms underlying the mechanical properties of fibrin fibers are elusive. We combined tensile testing of uncrosslinked fibrin polymers in vitro and in silico to explore their material properties. The experimental stress (σ) – strain (ε) curves for fibrin fibers are characterized by elastic deformations with a weaker elastic response for ε<160% due to unraveling of αC tethers and straightening of fibrin protofibrils, and a stronger response for ε>160% owing to unfolding of the coiled coils and γ nodules in fibrin monomers. Fiber rupture for strains ε>212% is due to dissociation of the knob-hole bonds and rupture of D:D interfaces. We developed the Fluctuating Bilinear Spring model to interpret the σ – ε profiles in terms of the free energy for protofibril alignment ΔG0 = 10.1–11.5 kBT, Young’s moduli for protofibril alignment Yu = 1.9–3.2 MPa and stretching Ya = 5.7–9.7 MPa, strain scale for fiber rupture, and protofibril cooperativity m = 3.6–8. We applied the model to characterize the fiber strength σcr = 12–13 MPa, deformability εcr ≈ 222%, and rupture toughness U ≈ 9 MJ/m3, and to resolve thermodynamic state functions, 96.9 GJ/mol entropy change for protofibril alignment (at room temperature) and 113.6 GJ/mol enthalpy change for protofibril stretching, which add up to 210.5 GJ/mol free-energy change. Fiber elongation is associated with protofibril dehydration and sliding mechanism to create an ordered protofibril array. Fibrin fibers behave like a hydrogel; protofibril dehydration and water expulsion account for ~94–98% of the total free-energy changes for fiber elongation and rupture.
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影响因子：
2.9
作者：
Gorkun, OV;Henschen-Edman, AH;Lord, ST
通讯作者：
Lord, ST
DOI：
10.1161/01.atv.0000241589.52950.4c
发表时间：
2006-11-01
影响因子：
8.7
作者：
Collet, J. P.;Allali, Y.;Montalescot, G.
通讯作者：
Montalescot, G.
影响因子：
3.4
作者：
Helms, Christine C.;Ariens, Robert A. S.;Guthold, Martin
通讯作者：
Guthold, Martin
影响因子：
3.4
作者：
Hudson, Nathan E.;Houser, John R.;Falvo, Michael R.
通讯作者：
Falvo, Michael R.
影响因子：
5.7
作者：
Hyeon, Changbong;Dima, Ruxandra I.;Thirumalai, D.
通讯作者：
Thirumalai, D.