Three-dimensional distribution of nigrostriatal neurons in the rat: Relation to the topography of striatonigral projections
Three-dimensional distribution of nigrostriatal neurons in the rat: Relation to the topography of striatonigral projections
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DOI：
10.1016/s0306-4522(98)00681-2
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发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Deniau, JM
中科院分区：
文献类型：
--
作者：
Maurin, Y;Banrezes, B;Deniau, JM
与确定的皮质领土相关的大鼠纹状体的功能区域被注入离子电泳与小麦胚芽凝集素耦合辣根过氧化物酶。在整个黑质中切割冠状连续切片。研究了标记的纹状体投射和黑质纹状体神经元的分布。使用在我们的实验室开发的软件，三维重建进行了计算，证实和扩展的组织方案的纹状体黑质预测已经报告了我们的小组。这些预测被组织为一组纵向lamebrates空间组织，以便分离的信息流来自附属于感觉运动和关联边缘皮质区的纹状体区域。此外，利用三维重建技术研究了黑质纹状体投射与黑质纹状体神经元的关系。对于每个纹状体注射部位，两个群体的逆行标记的黑质神经元可以区分它们的位置相对于纹状体投影场。第一个占据了近端的位置，与标记的纹状体预测登记，而第二个是更远。支配纹状体不同功能区的近端神经元群在内侧、背腹侧和吻尾侧均分离，而远端神经元的群体更分散，显示出较小程度的空间分离。这两个群体相对于纹状体投射野的组织表明，黑质可能通过两种不同的方式控制皮质信息如何通过纹状体。模式，分别基于涉及近端神经元的闭合黑质纹状体回路和涉及远端神经元的开放回路。(C)1999年IBRO。出版社：Elsevier Science Ltd
Functional regions of the rat striatum related to identified cortical territories were injected ionophoretically with wheat germ agglutinin coupled to horseradish peroxidase. Coronal serial sections were cut throughout the substantia nigra. The distributions of labelled striatal projections and nigrostriatal neurons were studied. Using software developed in our laboratory, three-dimensional reconstructions were calculated which confirmed and extended the organizational scheme of striatonigral projections already reported by our group. These projections were organized as a set of longitudinal lamellae spatially organized so as to segregate the flow of information emanating from striatal regions affiliated to sensorimotor and associative-limbic cortical areas. In addition, the relationship between the striatonigral projections and the nigrostriatal neurons was studied by three-dimensional reconstruction. For each striatal injection site, two populations of retrogradely labelled nigral neurons could be discriminated by their position with respect to the striatal projection field. The first one occupied a proximal position, in register with the labelled striatal projections, while the second was more distal. The populations of proximal neurons which innervate different functional striatal sectors were segregated both mediolaterally, dorsoventrally and rostrocaudally, while the populations of distal neurons were more scattered and showed a lesser degree of spatial segregation.The organization of these two populations with respect to the striatal projection fields suggests that the substantia nigra might control the how of cortical information through the striatum via two different modalities, based respectively on a closed nigrostriatal loop involving the proximal neurons, and an open loop involving the distal ones. (C) 1999 IBRO. Published by Elsevier Science Ltd.