DNA FINGER-PRINTING TO DISTINGUISH CULTIVAR AND PARENTAL RELATION OF JAPANESE PEAR
DNA FINGER-PRINTING TO DISTINGUISH CULTIVAR AND PARENTAL RELATION OF JAPANESE PEAR
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DOI：
10.2503/jjshs.63.17
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发表时间：
1994-06-01
影响因子：
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通讯作者：
KAMIYAMA, K
中科院分区：
文献类型：
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作者：
TERAMOTO, S;KANOMURAKAMI, Y;KAMIYAMA, K
通过限制性片段长度多态性（RFLP）的“DNA指纹”可以通过亲子关系分析来区分单个梨品种，这在许多其他生物体（包括人类）中得到了证明。利用人类小卫星DNA探针（33.6。33. 15) .通过对8个日本梨杂交种的分析，证明了‘丰水’不是‘梨14’ב八云’的杂种。
'DNA finger-printing' by Restriction Fragments Length Polymorphisms (RFLPs) can distinguish individual pear cultivars by a paternity analysis as demonstrated in many other organisms, including humans. In this study, 17 cultivars of Japanese pear were identified from their specific hybridization patterns using human minisatellite DNA probes (33.6. 33. 15) . By using these analysis, eight hybrid Japanese pears were traced to their parents; 'Housui' was proven not to be a hybrid of 'Ri-14' X 'Yakumo'.