Danggui-Shaoyao-San Improves Learning and Memory in Female SAMP8 via Modulation of Estradiol.
Danggui-Shaoyao-San Improves Learning and Memory in Female SAMP8 via Modulation of Estradiol.
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当归芍药散通过调节雌二醇改善女性 SAMP8 的学习和记忆
DOI：
10.1155/2014/327294
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhang YX
中科院分区：
文献类型：
--
作者：
Huang Y;Hu ZY;Yuan H;Shu L;Liu G;Qiao SY;Sun L;Zhou WX;Zhang YX
既往研究表明，当归芍药散（DSS）能改善阿尔茨海默病（AD）患者的认知功能障碍。然而，其机制仍不清楚;我们现在已经研究了DSS对SAMP 8的影响，并阐明了可能的机制。给药2个月后，采用跳台实验和Morris水迷宫实验评价大鼠的认知能力。检测血浆和海马组织中雌二醇（E2）、一氧化氮（NO）和甘氨酸的含量，探讨其可能的作用机制。SAMP 8在跳台试验中的潜伏期比年龄匹配的SAMR 1短，DSS使潜伏期延长，尤其是雌性动物。在MWM测试中，我们得到了类似的结果，SAMP 8花费更多的时间来找到平台，DSS减少了找到平台之前的时间，对游泳速度的影响不大。在测试期间，DSS增加了花费在目标象限的时间，特别是在女性SAMP 8。在雌性SAMP 8中，血浆E2、NO和血浆或海马组织中的甘氨酸升高。结论：DSS可改善SAMP 8小鼠认知功能的下降，尤其是雌性动物。增加E2、NO和甘氨酸可能有助于DSS对女性SAMP 8的认知改善作用。
Previous studies showed that Danggui-Shaoyao-San (DSS), a traditional Chinese medicinal prescription, could alleviate cognitive dysfunction of Alzheimer's disease (AD) patients. However, the mechanisms remain unclear; we have now examined the effect of DSS on SAMP8 and elucidated the possible mechanism. Animals were treated with DSS for 2 months, and step-down test and Morris water maze (MWM) test were used to evaluated cognitive abilities. The estradiol (E2), NO, and glycine in blood plasma or in hippocampus were detected to explore the possible mechanisms. The latency of SAMP8 in step-down test was shorter than that of age-matched SAMR1, and DSS increased the latency especially in female animals. In MWM test, we got similar results; SAMP8 spent more time to find the platform, and DSS decreased the time before finding the platform, with little effect on swim velocity, during the training sessions. During test session, DSS increased the time spent in target quadrant especially in female SAMP8. In female SAMP8, plasma E2, NO, and glycine were elevated in plasma or hippocampus tissue. In conclusion, DSS could ameliorate deterioration of cognition in SAMP8, especially in female animals. Increasing E2, NO, and glycine might contribute to the cognitive improvement effect of DSS in female SAMP8.